Number of the records: 1
Long-Term IgG Response to Porcine Neu5Gc Antigens without Transmission of PERV in Burn Patients Treated with Porcine Skin Xenografts
- 1.
SYSNO ASEP 0423300 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Long-Term IgG Response to Porcine Neu5Gc Antigens without Transmission of PERV in Burn Patients Treated with Porcine Skin Xenografts Author(s) Scobie, L. (GB)
Padler-Karavani, V. (US)
Les Bas-Bernardet, S. (FR)
Crossan, C. (GB)
Bláha, J. (CZ)
Matoušková, Magda (UMG-J)
Hector, R.D. (GB)
Cozzi, E. (IT)
Vanhove, B. (FR)
Charreau, B. (FR)
Blancho, G. (FR)
Bourdais, L. (FR)
Tallacchini, M. (IT)
Ribes, J. (GB)
Yu, H. (US)
Chen, X. (US)
Kracíková, J. (CZ)
Brož, L. (CZ)
Hejnar, Jiří (UMG-J) RID
Veselý, Pavel (UMG-J)
Takeuchi, Y. (GB)
Varki, A. (US)
Soulillou, J.P. (FR)Source Title Journal of Immunology. - : American Association of Immunologists - ISSN 0022-1767
Roč. 191, č. 6 (2013), s. 2907-2915Number of pages 9 s. Language eng - English Country US - United States Keywords PERV transmission ; xenotransplantation ; long-term immune response Subject RIV EB - Genetics ; Molecular Biology Next source Framework programmes of European Commission UT WOS 000324206900008 DOI 10.4049/jimmunol.1301195 Annotation Acellular materials of xenogenic origin are used worldwide as xenografts, and phase I trials of viable pig pancreatic islets are currently being performed. However, limited information is available on transmission of porcine endogenous retrovirus (PERV) after xenotransplantation and on the long-term immune response of recipients to xenoantigens. We analyzed the blood of burn patients who had received living pig-skin dressings for up to 8 wk for the presence of PERV as well as for the level and nature of their long term (maximum, 34 y) immune response against pig Ags. Although no evidence of PERV genomic material or anti-PERV Ab response was found, we observed a moderate increase in anti-alpha Gal Abs and a high and sustained anti-non-alpha Gal IgG response in those patients. Abs against the nonhuman sialic acid Neu5Gc constituted the anti-non-alpha Gal response with the recognition pattern on a sialoglycan array differing from that of burn patients treated without pig skin. These data suggest that anti-Neu5Gc Abs represent a barrier for long-term acceptance of porcine xenografts. Because anti-Neu5Gc Abs can promote chronic inflammation, the long-term safety of living and acellular pig tissue implants in recipients warrants further evaluation. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2014
Number of the records: 1