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An unexpected gene cluster for downstream degradation of alkylphenols in Sphingomonas sp strain TTNP3
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SYSNO ASEP 0387875 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title An unexpected gene cluster for downstream degradation of alkylphenols in Sphingomonas sp strain TTNP3 Author(s) Kolvenbach, B.A. (CH)
Dobrowinski, H. (CH)
Fousek, Jan (UMG-J)
Vlček, Čestmír (UMG-J) RID
Schaffer, A. (DE)
Gabriel, F.L.P. (DE)
Kohler, H.P.E. (CH)
Corvini, P.F.X. (CH)Source Title Applied Microbiology and Biotechnology. - : Springer - ISSN 0175-7598
Roč. 93, č. 3 (2012), s. 1315-1324Number of pages 10 s. Language eng - English Country DE - Germany Keywords hydroquinone ; degradation ; Sphingomonas ; nonylphenol ; bisphenol A Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000300282500039 DOI 10.1007/s00253-011-3451-8 Annotation In silico analysis of nucleotide sequences flanking the recently found hydroquinone dioxygenase in Sphingomonas sp. strain TTNP3 revealed a gene cluster that encodes a hydroquinone catabolic pathway. In addition to the two open-reading frames encoding the recently characterized hydroquinone dioxygenase, the cluster consisted of six open-reading frames. We were able to express the three open-reading frames, hqdC, hqdD, and hqdE, and demonstrated that the three gene products, HqdC, HqdD, and HqdE had 4-hydroxymuconic semialdehyde dehydrogenase, maleylacetate reductase, and intradiol dioxygenase activity, respectively. Surprisingly, the gene cluster showed similarities to functionally related clusters found in members of the alpha- and gamma-proteobacteria rather than to those found in other members of the genus Sphingomonas sensu latu. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2013
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