Filtration microcartridge and capillary isoelectric focusing for the analysis low number of microorganisms in real samples

0386009 - ÚIACH 2013 RIV CZ eng C - Conference Paper (international conference)
Kubesová, Anna - Horká, Marie - Šalplachta, Jiří - Horký, J.
Filtration microcartridge and capillary isoelectric focusing for the analysis low number of microorganisms in real samples.
CECE 2012. 9th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012 - (Foret, F.; Křenková, J.; Guttman, A.; Klepárník, K.; Boček, P.), s. 225-227. ISBN 978-80-904959-1-3.
[CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./. Brno (CZ), 01.11.2012-02.11.2012]
R&D Projects: GA MV VG20112015021; GA MŠMT(CZ) EE2.3.20.0182
Institutional support: RVO:68081715
Keywords : capillary isoelectric focusing * pre-concentration of microorganisms * filtration
Subject RIV: CB - Analytical Chemistry, Separation

At present, detection and identification of microorganisms in real biological samples needs very sensitive methods for correct diagnosis and therapy in medical practice, biotechnology, agriculture, food safety and quality etc. Accordingly, it is essential to develop new cheap techniques for pathogen identification, particularly for pathogen in complex biological samples. Pre-concentration, separation, and sensitive detection of whole cells in one step are great potential and advantage for detection of pathogens in low concentration. Combination of filtration microcartridge and capillary isoelectric focusing was developed for pre-concentration and pre-separation of microorganisms from real suspensions and the possibility its application to real samples was verified.
Permanent Link: http://hdl.handle.net/11104/0215232