Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA

Number of the records: 1

1.

SYSNO ASEP	0383454
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA
Author(s)	Bartošík, Martin (BFU-R)_RID Fojta, Miroslav (BFU-R)_{RID, ORCID} Paleček, Emil (BFU-R)_{RID, ORCID}
Number of authors	3
Source Title	Electrochimica acta. - : Elsevier - ISSN 0013-4686 Roč. 78, SEP (2012), s. 75-81
Number of pages	7 s.
Language	eng - English
Country	NL - Netherlands
Keywords	DNA methylation ; 5-methylcytosine analysis ; sodium bisulfite
Subject RIV	BO - Biophysics
R&D Projects	GAP301/11/2055 GA ČR - Czech Science Foundation (CSF)
	ME09038 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50040702 - BFU-R (2007-2013)
UT WOS	000308259500010
DOI	10.1016/j.electacta.2012.05.115
Annotation	Using DNA bisulfite treatment, reducible cytosines were transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks of unmethylated DNA. On the other hand, 5-methylcytosines were not transformed and the reduction peaks of methylated DNA remained relatively unchanged. By combining DNA bisulfite treatment with square wave voltammetry, DNA methylation can be determined quantitatively at nanomolar and subnanomolar ODN concentrations.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2013

Number of the records: 1