Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

0383204 - ÚEM 2013 RIV CZ eng J - Journal Article
Plánka, L. - Srnec, R. - Rauser, P. - Starý, D. - Filová, Eva - Jančář, J. - Juhásová, Jana - Křen, J. - Nečas, A. - Gál, P.
Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs.
Biomedical Papers. Roč. 156, č. 2 (2012), s. 128-134. ISSN 1213-8118. E-ISSN 1804-7521
R&D Projects: GA MZd(CZ) NS9896
Institutional research plan: CEZ:AV0Z50390512; CEZ:AV0Z50450515
Institutional support: RVO:68378041 ; RVO:67985904
Keywords : mesenchymal stem cells * growth plate defect * bone bridge
Subject RIV: FI - Traumatology, Orthopedics
Impact factor: 0.990, year: 2012

This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes and a scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs. In the left femoral bones the scaddold seeded with both MSCs and chondrocytes was transplanted into the iatrogenic partial distal growth plate defect, and scaffold without cells into right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined. Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases.
Permanent Link: http://hdl.handle.net/11104/0213217