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Antiviral effect of HPMPC (Cidofovir (R)), entrapped in cationic liposomes: In vitro study on MDBK cell and BHV-1 virus

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    SYSNO ASEP0378945
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleAntiviral effect of HPMPC (Cidofovir (R)), entrapped in cationic liposomes: In vitro study on MDBK cell and BHV-1 virus
    Author(s) Korvasová, Z. (CZ)
    Drašar, L. (CZ)
    Mašek, J. (CZ)
    Turánek Knotigová, P. (CZ)
    Kulich, P. (CZ)
    Matiašovic, J. (CZ)
    Kovařčík, K. (CZ)
    Bartheldyová, E. (CZ)
    Koudelka, Š. (CZ)
    Škrabalová, M. (CZ)
    Miller, A. D. (GB)
    Holý, Antonín (UOCHB-X)
    Ledvina, Miroslav (UOCHB-X) RID
    Turánek, J. (CZ)
    Number of authors14
    Source TitleJournal of Controlled Release. - : Elsevier - ISSN 0168-3659
    Roč. 160, č. 2 (2012), s. 330-338
    Number of pages9 s.
    Languageeng - English
    CountryNL - Netherlands
    Keywordscationic lipids ; BHV-1 virus ; Cidofovir ; HPMC ; antiviral drugs
    Subject RIVCC - Organic Chemistry
    R&D ProjectsGAP304/10/1951 GA ČR - Czech Science Foundation (CSF)
    KAN200520703 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    KAN200100801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    CEZAV0Z40550506 - UOCHB-X (2005-2011)
    UT WOS000305788800027
    DOI10.1016/j.jconrel.2012.01.040
    AnnotationWe designed and synthesised a series of new cationic lipids based on spermine linked to various hydrophobic anchors. These lipids could be potentially useful for the preparation of stable cationic liposomes intended for the construction of drug targeting systems applicable in the field of anticancer/antiviral therapy, vaccine carriers, and vectors for the gene therapy. Low in vitro toxicity was found for these compounds, especially for LD1, in several cell lines. The delivery of both a fluorescence marker (calcein) and antiviral drugs into cells has been achieved owing to a large extent of internalization of cationic liposomes (labelled by Lyssamine-Rhodamine PE or fluorescein-PE) as demonstrated by fluorescent microscopy and quantified by flow cytometry. The bovine herpes virus type 1 (BHV-1) virus infection in vitromodel using MDBK cells was employed to study the effect of the established antiviral drug HPMPC (Cidofovir (R)) developed by Prof. A. Holy. Inhibition of BHV-1 virus replication was studied by quantitative RT-PCR and confirmed by both Hoffman modulation contrast microscopy and transmission electron microscopy. We found that in vitro antiviral activity of HPMPC was significantly improved by formulation in cationic liposomes, which decreased the viral replication by about 2 orders of magnitude.
    WorkplaceInstitute of Organic Chemistry and Biochemistry
    Contactasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
    Year of Publishing2013
Number of the records: 1  

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