Antiviral effect of HPMPC (Cidofovir (R)), entrapped in cationic liposomes: In vitro study on MDBK cell and BHV-1 virus

Korvasová, Z.; Drašar, L.; Mašek, J.; Turánek Knotigová, P.; Kulich, P.; Matiašovic, J.; Kovařčík, K.; Bartheldyová, E.; Koudelka, Š.; Škrabalová, M.; Miller, A. D.; Holý, Antonín; Ledvina, Miroslav; Turánek, J.

doi:https://dx.doi.org/10.1016/j.jconrel.2012.01.040

Number of the records: 1

Antiviral effect of HPMPC (Cidofovir (R)), entrapped in cationic liposomes: In vitro study on MDBK cell and BHV-1 virus

1.

SYSNO ASEP	0378945
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Antiviral effect of HPMPC (Cidofovir (R)), entrapped in cationic liposomes: In vitro study on MDBK cell and BHV-1 virus
Author(s)	Korvasová, Z. (CZ) Drašar, L. (CZ) Mašek, J. (CZ) Turánek Knotigová, P. (CZ) Kulich, P. (CZ) Matiašovic, J. (CZ) Kovařčík, K. (CZ) Bartheldyová, E. (CZ) Koudelka, Š. (CZ) Škrabalová, M. (CZ) Miller, A. D. (GB) Holý, Antonín (UOCHB-X) Ledvina, Miroslav (UOCHB-X)_RID Turánek, J. (CZ)
Number of authors	14
Source Title	Journal of Controlled Release. - : Elsevier - ISSN 0168-3659 Roč. 160, č. 2 (2012), s. 330-338
Number of pages	9 s.
Language	eng - English
Country	NL - Netherlands
Keywords	cationic lipids ; BHV-1 virus ; Cidofovir ; HPMC ; antiviral drugs
Subject RIV	CC - Organic Chemistry
R&D Projects	GAP304/10/1951 GA ČR - Czech Science Foundation (CSF)
	KAN200520703 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	KAN200100801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000305788800027
DOI	10.1016/j.jconrel.2012.01.040
Annotation	We designed and synthesised a series of new cationic lipids based on spermine linked to various hydrophobic anchors. These lipids could be potentially useful for the preparation of stable cationic liposomes intended for the construction of drug targeting systems applicable in the field of anticancer/antiviral therapy, vaccine carriers, and vectors for the gene therapy. Low in vitro toxicity was found for these compounds, especially for LD1, in several cell lines. The delivery of both a fluorescence marker (calcein) and antiviral drugs into cells has been achieved owing to a large extent of internalization of cationic liposomes (labelled by Lyssamine-Rhodamine PE or fluorescein-PE) as demonstrated by fluorescent microscopy and quantified by flow cytometry. The bovine herpes virus type 1 (BHV-1) virus infection in vitromodel using MDBK cells was employed to study the effect of the established antiviral drug HPMPC (Cidofovir (R)) developed by Prof. A. Holy. Inhibition of BHV-1 virus replication was studied by quantitative RT-PCR and confirmed by both Hoffman modulation contrast microscopy and transmission electron microscopy. We found that in vitro antiviral activity of HPMPC was significantly improved by formulation in cationic liposomes, which decreased the viral replication by about 2 orders of magnitude.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
Year of Publishing	2013

Number of the records: 1