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Synthesis of Multivalent Glycoconjugates Containing the Immunoactive LELTE Peptide: Effect of Glycosylation on Cellular Activation and Natural Killing by Human Peripheral Blood Mononuclear Cells
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SYSNO ASEP 0348154 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Synthesis of Multivalent Glycoconjugates Containing the Immunoactive LELTE Peptide: Effect of Glycosylation on Cellular Activation and Natural Killing by Human Peripheral Blood Mononuclear Cells Author(s) Renaudet, O. (FR)
Křenek, Karel (MBU-M)
Bossu, I. (FR)
Dumy, P. (FR)
Kádek, A. (CZ)
Adámek, David (MBU-M)
Vaněk, O. (CZ)
Kavan, Daniel (MBU-M) RID, ORCID
Gažák, Radek (MBU-M) RID, ORCID
Šulc, Miroslav (MBU-M) RID, ORCID
Bezouška, K. (CZ)
Křen, Vladimír (MBU-M) RID, ORCIDSource Title Journal of the American Chemical Society. - : American Chemical Society - ISSN 0002-7863
Roč. 132, č. 19 (2010), s. 6800-6808Number of pages 9 s. Language eng - English Country US - United States Keywords KILLER-CELLS ; TN ANTIGEN ; RECEPTOR Subject RIV EE - Microbiology, Virology R&D Projects LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA400200503 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA303/09/0477 GA ČR - Czech Science Foundation (CSF) GD305/09/H008 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000277721500040 DOI 10.1021/ja101296t Annotation Pentapeptide diacidic sequence LELTE, derived from the mycobacterial heat shock protein hsp65, has been recently identified as a “danger” signal of the immune system effective via specific binding to the universal leukocyte triggering receptor CD69. This sequence is not active per se, only after its presentation within the multivalent environment of its parent protein, or after artificial dimerization using a standard bifunctional reagents. Here we describe an entirely new way of presenting of this peptide based on its attachment to a cyclopeptide RAFT scaffold (K-K-K-P-G)2 through the ε-amino group of lysine residues, alone or in combination with the carbohydrate epitope αGalNAc. The ability of such RAFT scaffolds to precipitate the target CD69 receptor or to activate CD69-positive cells is enhanced in compounds 2 and 4 possessing combined peptide/carbohydrate expression. Compounds 2 and 4 are highly efficient activators of natural killer lymphocytes Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
Number of the records: 1