The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic

0339342 - BC 2010 RIV BE eng C - Conference Paper (international conference)
Navrátil, M. - Šafářová, D. - Gadiou, S. - Fránová, Jana - Kučerová, J. - Talacko, L.
The partial molecular characterization of Plum pox virus infecting sweet cherry trees in the Czech Republic.
Proceedings of the twentieth international symposium on virus and virus-liked diseases of temperature fruit crops-fruit tree diseases. Leueven 1: ISHS Acta Horticulturea, 2008, s. 203-208. ISBN 978-90-6605-080-8. ISSN 0567-7572.
[International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops /20./. Antalya (TR), 22.05.2006-26.05.2006]
Institutional research plan: CEZ:AV0Z50510513
Keywords : Plum pox virus * Prunus avium * sweet cherry
Subject RIV: EB - Genetics ; Molecular Biology

The presence of PPV in sweet cherry (Prunus avium) has been previously reported within an experimental collection of cherry genotypes in Holovousy, Czech Republic. In this paper, the modified procedure of RNA isolation was successfully applied - one cycle of differential ultracentrifugation was inserted before standard RNA isolation using TRI Reagent. Five PPV virus isolates were detected by RT-PCR by commonly used P1/P2 primers. The strain specific PCR and sequence analysis of NIb-CP-3'NCR region (1375 bp length) of PPV isolate 13/2 CZ identified that isolate as member of PPV-Rec group. Studies are in progress to confirm biological transmission of the isolate.
Permanent Link: http://hdl.handle.net/11104/0182896