Elimination of hop latent viroid upon developmental activation of pollen nucleases

0321563 - BC 2009 RIV DE eng J - Journal Article
Matoušek, Jaroslav - Orctová, Lidmila - Škopek, Josef - Pešina, Karel - Steger, G.
Elimination of hop latent viroid upon developmental activation of pollen nucleases.
[Eliminace latentního viroidu chmele po vývojové aktivaci pylových nukleáz.]
Biological Chemistry. Roč. 389, č. 7 (2008), s. 905-918. ISSN 1431-6730. E-ISSN 1437-4315
R&D Projects: GA AV ČR(CZ) 1QS500510558; GA ČR GA521/06/1149
Institutional research plan: CEZ:AV0Z50510513
Keywords : hop latent viroid * elimination
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.035, year: 2008

We describe the process of HLVd elimination during development of hop pollen. HLVd propagates in uninucleate hop pollen, but is eliminated at stages following first pollen mitosis during pollen vacuolization and maturation. Only traces of HLVd were detected by RT-PCR in mature pollen after anthesis and no viroid was detectable in in vitro germinating pollen, suggesting complete degradation of circular and linear HLVd forms.

Je popsán proces eliminace HLVd během vývoje pylu chmelu. HLVd je obsažen v jednojaderném pylu, ale je eliminován po první pylové mitóze během vakuolizace a maturace. Pomocí RT-PCR byly detekovány pouhé stopy HLVd ve zralém pylu a vůbec žádný viroid v in vitro klíčícím pylu, což naznačuje kompletní degradaci jak cirkulární tak lineární formy HLVd.
Permanent Link: http://hdl.handle.net/11104/0170055