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Glycerophosphate-supported reactive oxygen species production in mammalian mitochondria
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SYSNO ASEP 0320988 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Glycerophosphate-supported reactive oxygen species production in mammalian mitochondria Title Glycerofosfát dependentní tvorba ROS v savčích mitochondriích Author(s) Vrbacký, Marek (FGU-C) RID, ORCID
Drahota, Zdeněk (FGU-C) RID, ORCID
Mráček, Tomáš (FGU-C) RID, ORCID
Pecinová, Alena (FGU-C) RID, ORCID, SAI
Houštěk, Josef (FGU-C) RID, ORCIDSource Title Bari International Symposium on Mitochondrial Physiology and Pathology. - Bari : University of Bari, 2008
SC3.14-SC3.14Number of pages 1 s. Action IUBMB Symposium S1 Event date 22.06.2008-26.06.2008 VEvent location Bari Country IT - Italy Event type WRD Language eng - English Country IT - Italy Keywords spo2 ; ROS ; mitochondria ; glycerol-3-phosphate dehydrogenase Subject RIV ED - Physiology R&D Projects 1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA303/06/1261 GA ČR - Czech Science Foundation (CSF) Next source Framework programmes of European Commission CEZ AV0Z50110509 - FGU-C (2005-2011) Annotation We demonstrate that mammalian mitochondrial glycerol-3-phosphate dehydrogenase (mGPDH, EC 1.1.99.5) represents an efficient site within mitochondrial respiratory chain where ROS could be formed. ROS-sensitive spectrofluorometric probes in the presence of specific respiratory chain inhibitors allowed us to analyze the contribution of dehydrogenase and coenzyme Q pool in ROS production as compared to complex III. In addition to the brown adipose tissue mitochondria that contain high amount of mGPDH, we investigated the ROS production in mitochondria from tissues, whose mGPDH content is very low (liver, heart, brain) and observed notable efficiency to generate ROS when glycerol-3-phosphate is present Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2009
Number of the records: 1