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Three-dimensional structure of a monomeric form of a retroviral protease
- 1.0194792 - UOCHB-X 20030098 RIV GB eng J - Journal Article
Veverka, V. - Bauerová, Helena - Zábranský, Aleš - Lang, J. - Ruml, Tomáš - Pichová, Iva - Hrabal, R.
Three-dimensional structure of a monomeric form of a retroviral protease.
Journal of Molecular Biology. Roč. 333, - (2003), s. 771-780. ISSN 0022-2836. E-ISSN 1089-8638
R&D Projects: GA ČR GA203/00/1241; GA AV ČR IAA4055304
Institutional research plan: CEZ:AV0Z4055905
Keywords : retroviral protease * monomer * M-PMV
Subject RIV: CE - Biochemistry
Impact factor: 5.239, year: 2003
Here we present a high resolution NMR structure of a fully folded monomer of a 12 kDa M-PMV protease (wt 12 PR) and of a Cys7Ala/Asp26Asn/Cys 106Ala mutant (12 PR D26N/C7A/C106A). We also show that cysteines C7 and C106 play a crucial role in stabilizing the dimer and consequently increasing the proteolytic activity of M-PMV PR.
Permanent Link: http://hdl.handle.net/11104/0090463
Number of the records: 1