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A miniaturized multichamber solution isoelectric focusing device for separation of protein digests
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SYSNO ASEP 0162432 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title A miniaturized multichamber solution isoelectric focusing device for separation of protein digests Author(s) Tan, A. (US)
Pashkova, A. (US)
Zang, L. (US)
Foret, František (UIACH-O) RID, ORCID
Karger, B. L. (US)Source Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 23, č. 20 (2002), s. 3599-3607Number of pages 8 s. Language eng - English Country DE - Germany Keywords solution IEF ; peptides ; mass spectrometry Subject RIV CB - Analytical Chemistry, Separation R&D Projects IBS4031209 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z4031919 - UIACH-O Annotation A miniaturized multichamber device was constructed for solution isoelectric focusing (IEF) separation of complex peptide mixtures. A variety of standard peptide mixtures and tryptic digests of proteins were separated. The IEF separation provided concentrated and desalted fractions, suitable for an additional separation liquid chromatography, capillary electrophoresis (LC, CE) or mass spectrometry (MS) detection without additional sample cleanup. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2003
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