0600331 - MBÚ 2025 RIV DE eng J - Journal Article
Němečková, K. - Mareš, Jan - Košek, F. - Culka, A. - Dudák, J. - Tymlová, V. - Žemlička, J. - Jehlička, J.
Comparative analysis of cyanobacterial communities in gypsum outcrops: insights from sites in Israel and Poland.
Extremophiles. Roč. 28, č. 3 (2024), č. článku 37. ISSN 1431-0651. E-ISSN 1433-4909
Institutional support: RVO:61388971
Keywords : atacama desert implications * microbial colonization * endolithic communities * hyperarid core * life * search * halite * rock * microorganisms * diversity * Phototrophs * Cyanobacteria * Endoliths * Gypsum * 16S rRNA * Metacommunity
OECD category: Microbiology
Impact factor: 2.6, year: 2023 ; AIS: 0.491, rok: 2023
Method of publishing: Open access
Result website:
https://link.springer.com/article/10.1007/s00792-024-01352-4DOI: https://doi.org/10.1007/s00792-024-01352-4

Today, the biodiversity of endolithic microbial colonisations are only partly understood. In this study, we used a combination of molecular community metabarcoding using the 16S rRNA gene, light microscopy, CT-scan analysis, and Raman spectroscopy to describe gypsum endolithic communities in 2 sites-southern Poland and northern Israel. The obtained results have shown that despite different geographical areas, climatic conditions, and also physical features of colonized gypsum outcrops, both of these sites have remarkably similar microbial and pigment compositions. Cyanobacteria dominate both of the gypsum habitats, followed by Chloroflexi and Pseudomonadota. Among cyanobacteria, Thermosynechococcaceae were more abundant in Israel while Chroococcidiopsidaceae in Poland. Interestingly, no Gloeobacteraceae sequences have been found in Poland, only in Israel. Some of the obtained 16S rRNA gene sequences of cyanobacteria matched previously detected sequences from endolithic communities in various substrates and geographical regions, supporting the hypothesis of global metacommunity, but more data are still needed. Using Raman spectroscopy, cyanobacterial UV-screening pigments-scytonemin and gloeocapsin have been detected alongside carotenoids, chlorophyll a and melanin. These pigments can serve as potential biomarkers for basic taxonomic identification of cyanobacteria. Overall, this study provides more insight into the diversity of cyanobacterial endolithic colonisations in gypsum across different areas.
Permanent Link: https://hdl.handle.net/11104/0357648