Number of the records: 1
An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome
- 1.
SYSNO ASEP 0584750 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome Author(s) Faria, J.R.C. (GB)
Tinti, M. (GB)
Marques, C.A. (GB)
Zoltner, M. (CZ)
Yoshikawa, H. (JP)
Field, Mark Christian (BC-A) ORCID
Horn, D. (GB)Number of authors 7 Article number 8200 Source Title Nature Communications. - : Nature Publishing Group
Roč. 14, č. 1 (2023)Number of pages 18 s. Language eng - English Country US - United States Keywords monoallelic expression ; kinetoplast dna ; cell-cycle ; brucei ; rna ; genome ; transcription ; genes ; efficiency ; complexes Subject RIV EA - Cell Biology OECD category Cell biology Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 001185617500006 EID SCOPUS 85179314622 DOI 10.1038/s41467-023-44043-y Annotation UPF1-like helicases play roles in telomeric heterochromatin formation and X-chromosome inactivation, and also in monogenic variant surface glycoprotein (VSG) expression via VSG exclusion-factor-2 (VEX2), a UPF1-related protein in the African trypanosome. We show that VEX2 associates with chromatin specifically at the single active VSG expression site on chromosome 6, forming an allele-selective connection, via VEX1, to the trans-splicing locus on chromosome 9, physically bridging two chromosomes and the VSG transcription and splicing compartments. We further show that the VEX-complex is multimeric and self-regulates turnover to tightly control its abundance. Using single cell transcriptomics following VEX2-depletion, we observed simultaneous derepression of many other telomeric VSGs and multi-allelic VSG expression in individual cells. Thus, an allele-selective, inter-chromosomal, and self-limiting VEX1-2 bridge supports monogenic VSG expression and multi-allelic VSG exclusion. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2024 Electronic address https://www.nature.com/articles/s41467-023-44043-y#citeas
Number of the records: 1