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An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome
- 1.0584750 - BC 2024 RIV US eng J - Journal Article
Faria, J.R.C. - Tinti, M. - Marques, C.A. - Zoltner, M. - Yoshikawa, H. - Field, Mark Christian - Horn, D.
An allele-selective inter-chromosomal protein bridge supports monogenic antigen expression in the African trypanosome.
Nature Communications. Roč. 14, č. 1 (2023), č. článku 8200. E-ISSN 2041-1723
Institutional support: RVO:60077344
Keywords : monoallelic expression * kinetoplast dna * cell-cycle * brucei * rna * genome * transcription * genes * efficiency * complexes
OECD category: Cell biology
Impact factor: 16.6, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s41467-023-44043-y#citeas
UPF1-like helicases play roles in telomeric heterochromatin formation and X-chromosome inactivation, and also in monogenic variant surface glycoprotein (VSG) expression via VSG exclusion-factor-2 (VEX2), a UPF1-related protein in the African trypanosome. We show that VEX2 associates with chromatin specifically at the single active VSG expression site on chromosome 6, forming an allele-selective connection, via VEX1, to the trans-splicing locus on chromosome 9, physically bridging two chromosomes and the VSG transcription and splicing compartments. We further show that the VEX-complex is multimeric and self-regulates turnover to tightly control its abundance. Using single cell transcriptomics following VEX2-depletion, we observed simultaneous derepression of many other telomeric VSGs and multi-allelic VSG expression in individual cells. Thus, an allele-selective, inter-chromosomal, and self-limiting VEX1-2 bridge supports monogenic VSG expression and multi-allelic VSG exclusion.
Permanent Link: https://hdl.handle.net/11104/0352594
Number of the records: 1