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Undertaking the biological sex assessment of human remains: The applicability of minimally-invasive methods for proteomic sex estimation from enamel peptides
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SYSNO ASEP 0582102 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Undertaking the biological sex assessment of human remains: The applicability of minimally-invasive methods for proteomic sex estimation from enamel peptides Author(s) Brůžek, J. (CZ)
Mikšík, I. (CZ)
Kotěrová Pilmann, A. (CZ)
Morvan, Marine (FGU-C) RID, ORCID, SAI
Kaupová Drtikolová, S. (CZ)
Santos, F. (FR)
Danielisová, Alžběta (ARU-G) RID, SAI, ORCID
Zazvonilová, Eliška (ARU-G) ORCID, RID, SAI
Maureille, B. (FR)
Velemínský, P. (CZ)Source Title Journal of Cultural Heritage. - : Elsevier - ISSN 1296-2074
Roč. 66, March–April (2024), s. 204-214Number of pages 11 s. Language eng - English Country NL - Netherlands Keywords human skeletal collections ; cultural heritage ; proteomics ; sex estimation ; sampling OECD category Analytical chemistry Institutional support FGU-C - RVO:67985823 ; ARU-G - RVO:67985912 UT WOS 001135047200001 EID SCOPUS 85179078661 DOI 10.1016/j.culher.2023.11.021 Annotation Being a part of the cultural heritage, skeletal human remains and grave objects are often the only evidence of people who lived many years, or even centuries or millennia, ago, and their preservation for future generations is thus of the utmost importance. The first task in analyzing skeletal remains is to build a biological profile of the individual, including in particular a sex estimation. Recently developed proteomic sex analysis, based on the detection of two sex-dependent forms of the amelogenin protein in tooth enamel, could offer a minimally-invasive and reliable approach applicable to both recent and past populations. The aims of the present study are: 1) to validate the proteomic sex estimation approach with a delicate, minimally-destructive protocol using protein etching in recent and sub-recent identified samples of adult individuals, 2) for the first time, to evaluate the invasiveness of the extraction of amelogenin protein from teeth for proteomic analysis via scanning electron microscope (SEM) and microcomputed tomography (micro-CT), 3) to apply the method to an archaeological sample of unknown adult and juvenile individuals. An assemblage of 60 teeth (32 males and 28 females) of recent and sub-recent origin was used to validate the approach. A sub-sample of 20 teeth (10 males and 10 females) was used to assess the invasiveness of the amelogenin extraction procedure. For the application of the method, samples of 15 adult and 32 juvenile teeth, both originating from medieval populations, were used. Proteomic sex estimation achieved 100% accuracy in this sample. An SEM and micro-CT comparison of the dental surfaces before and after chemical treatment showed an approximately 10% loss of enamel and only 2% loss of dentine. The suitability and minimally-invasive character of the protocol for proteomic analysis in biological sex estimation was demonstrated, as was its applicability to archaeological samples. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2025 Electronic address https://doi.org/10.1016/j.culher.2023.11.021
Number of the records: 1