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The opportunistic protist, iGiardia intestinalis/i, occurs in gut-healthy humans in a high-income country
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SYSNO ASEP 0579876 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The opportunistic protist, iGiardia intestinalis/i, occurs in gut-healthy humans in a high-income country Author(s) Brožová, Kristýna (BC-A) SAI
Jirků, Milan (BC-A) RID
Lhotská, Zuzana (BC-A) RID, ORCID
Květoňová, Dana (BC-A) SAI
Kadlecová, Oldřiška (BC-A) SAI, RID
Stensvold, C.R. (DK)
Samaš, P. (CZ)
Petrželková, Klára Judita (BC-A) RID
Jirků-Pomajbíková, Kateřina (BC-A) ORCID, RIDNumber of authors 9 Article number 2270077 Source Title Emerging Microbes & Infections . - : Nature Publishing Group
Roč. 12, č. 2 (2023)Number of pages 10 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords molecular epidemiology ; entamoeba-histolytica ; duodenalis ; prevalence ; lamblia ; gene ; Giardia intestinalis, human volunteers ; qPCR ; conventional-PCR ; quantification ; contact with animals Subject RIV FP - Other Medical Disciplines OECD category Parasitology R&D Projects GA22-04837S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 001099095300001 EID SCOPUS 85175245819 DOI 10.1080/22221751.2023.2270077 Annotation Giardia intestinalis, a cosmopolitan gastrointestinal protist, is detected mainly in patients with clinical giardiasis in high-income countries. In contrast, there is very little information on the presence of Giardia in asymptomatic individuals. Therefore, the aim of this study was to determine the presence and prevalence of Giardia in gut-healthy volunteers in the Czech Republic and to perform a comparative evaluation of different diagnostic methods, since Giardia diagnostics is complicated. Our results confirmed that the qPCR method is the most sensitive method for detecting Giardia and revealed a prevalence of 7% (22/296) in asymptomatic individuals. In most cases, the colonization intensity ranged from 10(-1)-10(1). A conventional PCR protocol targeting the TPI gene was used to identify the assemblages. However, this protocol had limited sensitivity for Giardia amplification, effectively detecting colonization above an intensity of 104. In addition, Giardia was detected in 19% of the animals, which were closely associated with the study participants. However, due to methodological limitations, zoonotic transmission could not be clearly confirmed. Notably, contact with animals proved to be the only factor that had a significant impact on the incidence of Giardia in gut-healthy humans. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2024 Electronic address https://www.tandfonline.com/doi/full/10.1080/22221751.2023.2270077
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