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Key Engineering Materials
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SYSNO ASEP 0577507 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title PCL scaffold for osteochondral defect treatment Author(s) Blahnová, Veronika (UEM-P)
Vocetková, Karolína (UEM-P) ORCID
Hlinková, Jana (UEM-P)
Divín, Radek (UEM-P)
Amler, E. (CZ)
Filová, Eva (UEM-P) RID, ORCIDSource Title Key Engineering Materials. - Bäch : Scientific, 2020 - ISBN 978-303571519-4 Pages s. 141-147 Number of pages 7 s. Number of pages 196 Publication form Print - P Language eng - English Country CH - Switzerland Keywords growth factors ; mesenchymal stem cells ; osteochondral defect OECD category Biomaterials (as related to medical implants, devices, sensors) R&D Projects GA18-09306S GA ČR - Czech Science Foundation (CSF) LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEM-P - RVO:68378041 EID SCOPUS 85083362985 DOI 10.4028/www.scientific.net/KEM.834.141 Annotation Osteochondral defects develop as a result of trauma, microtrauma, avascular necrosis or cancer. These are usually pre-arthrotic conditions, accompanied by chronic pain and limited joint mobility leading to decreased quality of life of the affected patients. The bone itself has self-repair potential facilitated by mesenchymal stem cells and other cells present in the bone tissue. On the other hand, mature cartilage has very low regenerative capacity due to limited mitotic potential of chondrocytes and lack of vascularization. Therefore, there is an effort to develop an alternative treatment strategy supporting and accelerating natural healing processes. We have designed nanofibrous scaffolds made of poly-ε-caprolactone/hyaluronic acid and enriched with specific growth factors - “osteogenic” part with BMP-2 and “chondrogenic” part with bFGF and TGF-β. These two parts are meant to be combined in one biphasic non-cellular scaffold which would be possible to implant in the site of injury and serve as a mechanical support for the cells. We examined proliferation and viability of cells, depth of their penetration into scaffold, cell distribution, alkaline phosphatase activity and extracellular matrix proteins expression. We showed both “osteogenic” and “chondrogenic” scaffold was suitable for cell growth. Moreover, in comparison to the control samples, these two scaffolds exhibited positive effect on chondrogenic and osteogenic differentiation, respectively. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2024 Electronic address https://www.scientific.net/KEM.834.141
Number of the records: 1