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Comprehensive ECG reference intervals in C57BL/6N substrains provide a generalizable guide for cardiac electrophysiology studies in mice
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SYSNO ASEP 0574410 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comprehensive ECG reference intervals in C57BL/6N substrains provide a generalizable guide for cardiac electrophysiology studies in mice Author(s) Oestereicher, M. A. (DE)
Wotton, J. M. (US)
Ayabe, S. (JP)
Bou About, G. (FR)
Cheng, T. K. (GB)
Choi, J. (KR)
Clary, D. (US)
Dew, E. M. (US)
Elfertak, L. (FR)
Guimond, A. (FR)
Haseli Mashhadi, H. (GB)
Heaney, J. D. (US)
Kelsey, L. (CA)
Keskivali-Bond, P. (US)
Lopez Gomez, F. (GB)
Marschall, S. (DE)
McFarland, M. (US)
Meziane, H. (FR)
Munoz Fuentes, V. (GB)
Nam, K. (KR)
Nichtová, Zuzana (UMG-J)
Pimm, D. (GB)
Bower, L. (US)
Procházka, Jan (UMG-J) ORCID
Rozman, Jan (UMG-J)
Santos, L. (GB)
Stewart, M. (GB)
Tanaka, N. (JP)
Ward, Ch. S. (US)
Willett, A. M. E. (US)
Sedláček, Radislav (UMG-J) RIDNumber of authors 51 Source Title Mammalian Genome. - : Springer - ISSN 0938-8990
Roč. 34, č. 2 (2023), s. 180-199Number of pages 20 s. Language eng - English Country DE - Germany Keywords Electrocardiography ; Electrophysiologic Techniques ; Inbred C57BL ; C57BL mouse ; heart function test OECD category Biochemistry and molecular biology R&D Projects ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015040 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018126 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001789 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0015861 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 001011019700001 EID SCOPUS 85161449409 DOI 10.1007/s00335-023-09995-y Annotation Reference ranges provide a powerful tool for diagnostic decision-making in clinical medicine and are enormously valuable for understanding normality in pre-clinical scientific research that uses in vivo models. As yet, there are no published reference ranges for electrocardiography (ECG) in the laboratory mouse. The first mouse-specific reference ranges for the assessment of electrical conduction are reported herein generated from an ECG dataset of unprecedented scale. International Mouse Phenotyping Consortium data from over 26,000 conscious or anesthetized C57BL/6N wildtype control mice were stratified by sex and age to develop robust ECG reference ranges. Interesting findings include that heart rate and key elements from the ECG waveform (RR-, PR-, ST-, QT-interval, QT corrected, and QRS complex) demonstrate minimal sexual dimorphism. As expected, anesthesia induces a decrease in heart rate and was shown for both inhalation (isoflurane) and injectable (tribromoethanol) anesthesia. In the absence of pharmacological, environmental, or genetic challenges, we did not observe major age-related ECG changes in C57BL/6N-inbred mice as the differences in the reference ranges of 12-week-old compared to 62-week-old mice were negligible. The generalizability of the C57BL/6N substrain reference ranges was demonstrated by comparison with ECG data from a wide range of non-IMPC studies. The close overlap in data from a wide range of mouse strains suggests that the C57BL/6N-based reference ranges can be used as a robust and comprehensive indicator of normality. We report a unique ECG reference resource of fundamental importance for any experimental study of cardiac function in mice. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2024 Electronic address https://link.springer.com/article/10.1007/s00335-023-09995-y
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