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Protracted morphine withdrawal induces upregulation of peroxiredoxin II and reduces 14-3-3 protein levels in the rat brain cortex and hippocampus
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SYSNO ASEP 0573778 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Protracted morphine withdrawal induces upregulation of peroxiredoxin II and reduces 14-3-3 protein levels in the rat brain cortex and hippocampus Author(s) Ujčíková, Hana (FGU-C) RID, ORCID
Hejnová, L. (CZ)
Novotný, J. (CZ)
Svoboda, Petr (FGU-C) RID, ORCIDArticle number 148428 Source Title Brain Research. - : Elsevier - ISSN 0006-8993
Roč. 1813, Aug 15 (2023)Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords protracted morphine withdrawal ; rat brain cortex ; rat hippocampus ; Peroxiredoxin II ; 14-3-3 proteins ; oxidative stress OECD category Physiology (including cytology) R&D Projects GA19-03295S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 001015314100001 EID SCOPUS 85160762624 DOI 10.1016/j.brainres.2023.148428 Annotation Protracted opioid withdrawal is considered to be a traumatic event with many adverse effects. However, little attention is paid to its consequences on the protein expression in the rat brain. A better understanding of the changes at the molecular level is essential for designing future innovative drug therapies. Our previous proteomic data indicated that long-term morphine withdrawal is associated with altered proteins functionally involved in energy metabolism, cytoskeletal changes, oxidative stress, apoptosis, or signal transduction. In this study, we selected peroxiredoxin II (PRX II) as a marker of oxidative stress, 14-3-3 proteins as adaptors, and creatine kinase-B (CK-B) as a marker of energy metabolism to detect their amounts in the brain cortex and hippocampus isolated from rats after 3-month (3 MW) and 6-month morphine withdrawal (6 MW). Methodically, our work was based on immunoblotting accompanied by 2D resolution of PRX II and 14-3-3 proteins. Our results demonstrate significant upregulation of PRX II in the rat brain cortex (3-fold) and hippocampus (1.3-fold) after 3-month morphine abstinence, which returned to the baseline six months since the drug was withdrawn. Interestingly, the level of 14-3-3 proteins was downregulated in both brain areas in 3 MW samples and remained decreased only in the brain cortex of 6 MW. Our findings suggest that the rat brain cortex and hippocampus exhibit the oxidative stress-induced vulnerability represented by compensatory upregulation of PRX II after three months of morphine withdrawal. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2024 Electronic address https://doi.org/10.1016/j.brainres.2023.148428
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