Interindividual differences contribute to variation in microbiota composition more than hormonal status: A prospective study

Jacková, Zuzana; Štěpán, J. J.; Coufal, Štěpán; Kostovčík, Martin; Galanová, Natálie; Reiss, Zuzana; Pavelka, K.; Wenchich, L.; Hrušková, H.; Kverka, Miloslav

doi:https://dx.doi.org/10.3389/fendo.2023.1139056

Number of the records: 1

Interindividual differences contribute to variation in microbiota composition more than hormonal status: A prospective study

1.

SYSNO ASEP	0571456
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Interindividual differences contribute to variation in microbiota composition more than hormonal status: A prospective study
Author(s)	Jacková, Zuzana (MBU-M) Štěpán, J. J. (CZ) Coufal, Štěpán (MBU-M)_{ORCID, RID} Kostovčík, Martin (MBU-M)_{RID, ORCID} Galanová, Natálie (MBU-M)_ORCID Reiss, Zuzana (MBU-M) Pavelka, K. (CZ) Wenchich, L. (CZ) Hrušková, H. (CZ) Kverka, Miloslav (MBU-M)_{RID, ORCID}
Article number	1139056
Source Title	Frontiers in Endocrinology. - : Frontiers Media - ISSN 1664-2392 Roč. 14, March 8 (2023)
Number of pages	15 s.
Language	eng - English
Country	CH - Switzerland
Keywords	microbiota ; hormonal therapy ; oophorectomy ; bone metabolism ; estrogen
OECD category	Microbiology
R&D Projects	NV18-05-00394 GA MZd - Ministry of Health (MZ)
Method of publishing	Open access
Institutional support	MBU-M - RVO:61388971
UT WOS	000963542300001
EID SCOPUS	85152568046
DOI	10.3389/fendo.2023.1139056
Annotation	AimsOvarian hormone deficiency is one of the main risk factors for osteoporosis and bone fractures in women, and these risks can be mitigated by menopausal hormone therapy. Recent evidence suggests that gut microbiota may link changes in estrogen levels and bone metabolism. This study was conducted to investigate the potential relationship between hormonal and bone changes induced by oophorectomy and subsequent hormonal therapy and shifts in gut microbiota composition. MethodsWe collected 159 stool and blood samples in several intervals from 58 women, who underwent bilateral oophorectomy. Changes in fecal microbiota were assessed in paired samples collected from each woman before and after oophorectomy or the start of hormone therapy. Bacterial composition was determined by sequencing the 16S rRNA gene on Illumina MiSeq. Blood levels of estradiol, FSH, biomarkers of bone metabolism, and indices of low-grade inflammation were measured using laboratory analytical systems and commercial ELISA. Areal bone mineral density (BMD) of the lumbar spine, proximal femur, and femur neck was measured using dual-energy X-ray absorptiometry. ResultsWe found no significant changes in gut microbiota composition 6 months after oophorectomy, despite major changes in hormone levels, BMD, and bone metabolism. A small decrease in bacterial diversity was apparent 18 months after surgery in taxonomy-aware metrics. Hormonal therapy after oophorectomy prevented bone loss but only marginally affected gut microbiota. There were no significant differences in beta-diversity related to hormonal status, although several microbes (e.g., Lactococcus lactis) followed estrogen levels. Body mass index (BMI) was the most significantly associated with microbiota variance. Microbiota was not a suitable predictive factor for the state of bone metabolism. ConclusionsWe conclude that neither the loss of estrogens due to oophorectomy nor their gain due to subsequent hormonal therapy is associated with a specific gut microbiota signature. Sources of variability in microbiota composition are more related to interindividual differences than hormonal status.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2024
Electronic address	https://www.frontiersin.org/articles/10.3389/fendo.2023.1139056/full

Number of the records: 1