Number of the records: 1
Early evolution of enamel matrix proteins is reflected by pleiotropy of physiological functions
- 1.
SYSNO ASEP 0571419 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Early evolution of enamel matrix proteins is reflected by pleiotropy of physiological functions Author(s) Špoutil, František (UMG-J)
Aranaz-Novaliches, Gorreti (UMG-J)
Procházková, Michaela (UMG-J)
Wald, T. (US)
Novosadová, Vendula (UMG-J)
Kašpárek, Petr (UMG-J)
Osička, Radim (MBU-M) RID, ORCID
Reseland, J. E. (NO)
Lyngstadaas, S. P. (NO)
Tiainen, H. (NO)
Boušová, Kristýna (UOCHB-X) ORCID
Vondrášek, Jiří (UOCHB-X) RID, ORCID
Sedláček, Radislav (UMG-J) RID
Procházka, Jan (UMG-J) ORCIDNumber of authors 14 Article number 1471 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 13, č. 1 (2023)Number of pages 14 s. Language eng - English Country US - United States Keywords Dental Enamel Proteins ; Animals ; Amelogenin ; Mice OECD category Biochemistry and molecular biology R&D Projects GJ19-19025Y GA ČR - Czech Science Foundation (CSF) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001789 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0015861 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018126 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018133 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CCP II - 90126 - Ústav molekulární genetiky AV ČR, v. v. i.
CCP III - 90236 - Ústav molekulární genetiky AV ČR, v. v. i.Method of publishing Open access Institutional support UMG-J - RVO:68378050 ; MBU-M - RVO:61388971 ; UOCHB-X - RVO:61388963 UT WOS 000970852200015 EID SCOPUS 85146845780 DOI 10.1038/s41598-023-28388-4 Annotation Highly specialized enamel matrix proteins (EMPs) are predominantly expressed in odontogenic tissues and diverged from common ancestral gene. They are crucial for the maturation of enamel and its extreme complexity in multiple independent lineages. However, divergence of EMPs occured already before the true enamel evolved and their conservancy in toothless species suggests that non-canonical functions are still under natural selection. To elucidate this hypothesis, we carried out an unbiased, comprehensive phenotyping and employed data from the International Mouse Phenotyping Consortium to show functional pleiotropy of amelogenin, ameloblastin, amelotin, and enamelin, genes, i.e. in sensory function, skeletal morphology, cardiovascular function, metabolism, immune system screen, behavior, reproduction, and respiratory function. Mice in all KO mutant lines, i.e. amelogenin KO, ameloblastin KO, amelotin KO, and enamelin KO, as well as mice from the lineage with monomeric form of ameloblastin were affected in multiple physiological systems. Evolutionary conserved motifs and functional pleiotropy support the hypothesis of role of EMPs as general physiological regulators. These findings illustrate how their non-canonical function can still effect the fitness of modern species by an example of influence of amelogenin and ameloblastin on the bone physiology. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2024 Electronic address https://www.nature.com/articles/s41598-023-28388-4
Number of the records: 1