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De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berries
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SYSNO ASEP 0568779 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berries Author(s) Sreekumar, Sweda (BC-A) RID, ORCID
Divya, K. (IN)
Joy, N. (IN)
Soniya, E. (IN)Number of authors 4 Article number 501 Source Title BMC Plant Biology. - : BioMed Central - ISSN 1471-2229
Roč. 22, č. 1 (2022)Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords Black pepper ; Fruits ; RNA-Seq ; Developmental stages ; Secondary metabolite Subject RIV EF - Botanics OECD category Plant sciences, botany Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000872034300003 EID SCOPUS 85140594228 DOI 10.1186/s12870-022-03878-1 Annotation Background Black pepper (Piper nigrum L.) is rich in bioactive compounds that make it an imperative constituent in traditional medicines. Although the unripe fruits have long been used in different Ayurvedic formulations, the mechanism of gene regulation resulting in the production of the bioactive compounds in black pepper is not much investigated. Exploring the regulatory factors favouring the production of bioactive compounds ultimately help to accumulate the medicinally important content of black pepper. The factors that enhance the biosynthesis of these compounds could be potential candidates for metabolic engineering strategies to obtain a high level production of significant biomolecules. Results Being a non-model plant, de novo sequencing technology was used to unravel comprehensive information about the genes and transcription factors that are expressed in mature unripe green berries of P. nigrum from which commercially available black pepper is prepared. In this study, the key gene regulations involved in the synthesis of bioactive principles in black pepper was brought out with a focus on the highly expressed phenylpropanoid pathway genes. Quantitative real-time PCR analysis of critical genes and transcription factors in the different developmental stages from bud to the mature green berries provides important information useful for choosing the developmental stage that would be best for the production of a particular bioactive compound. Comparison with a previous study has also been included to understand the relative position of the results obtained from this study. Conclusions The current study uncovered significant information regarding the gene expression and regulation responsible for the bioactivity of black pepper. The key transcription factors and enzymes analyzed in this study are promising targets for achieving a high level production of significant biomolecules through metabolic engineering. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2023 Electronic address https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-022-03878-1
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