Characterization of Endogenous Levels of Brassinosteroids and Related Genes in Grapevines

0565757 - ÚEB 2023 RIV CH eng J - Journal Article
Parada, F. - Oklešťková, Jana - Arce-Johnson, P.
Characterization of Endogenous Levels of Brassinosteroids and Related Genes in Grapevines.
International Journal of Molecular Sciences. Roč. 23, č. 3 (2022), č. článku 1827. E-ISSN 1422-0067
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Brassinosteroids * Development * Grapevine * uhplc-ms/ms
OECD category: Biochemistry and molecular biology
Impact factor: 5.6, year: 2022
Method of publishing: Open access
https://doi.org/10.3390/ijms23031827

Agronomic breeding practices for grapevines (Vitis vinifera L.) include the application of growth regulators in the field. Brassinosteroids (BRs) are a family of sterol-derived plant hormones that regulate several physiological processes and responses to biotic and abiotic stress. In grapevine berries, the production of biologically active BRs, castasterone and 6-deoxocastasterone, has been reported. In this work, key BR genes were identified, and their expression profiles were determined in grapevine. Bioinformatic homology analyses of the Arabidopsis genome found 14 genes associated with biosynthetic, perception and signaling pathways, suggesting a partial conservation of these pathways between the two species. The tissue-and development-specific expression profiles of these genes were determined by qRT-PCR in nine different grapevine tissues. Using UHPLC-MS/MS, 10 different BR compounds were pinpointed and quantified in 20 different tissues, each presenting specific accumulation patterns. Although, in general, the expression profile of the biosynthesis pathway genes of BRs did not directly correlate with the accumulation of metabolites, this could reflect the complexity of the BR biosynthesis pathway and its regulation. The development of this work thus generates a contribution to our knowledge about the presence, and diversity of BRs in grapevines.
Permanent Link: https://hdl.handle.net/11104/0337266