Number of the records: 1
The role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions
- 1.
SYSNO ASEP 0565356 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The role of polyhydroxyalkanoates in adaptation of Cupriavidus necator to osmotic pressure and high concentration of copper ions Author(s) Nováčková, I. (CZ)
Hrabalová, V. (CZ)
Slaninová, E. (CZ)
Sedláček, P. (CZ)
Samek, Ota (UPT-D) RID, ORCID, SAI
Koller, M. (AT)
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Mrázová, Kateřina (UPT-D) SAI, ORCID, RID
Nebesářová, Jana (BC-A) RID, ORCID
Obruča, S. (CZ)Number of authors 11 Source Title International Journal of Biological Macromolecules. - : Elsevier - ISSN 0141-8130
Roč. 206, 1 May (2022), s. 977-989Number of pages 13 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords Adaptation ; Cupriavidus necator ; Heavy metals ; Osmotic stress ; Polyhydroxyalkanoates ; Stress conditions Subject RIV JB - Sensors, Measurment, Regulation OECD category Electrical and electronic engineering Subject RIV - cooperation Biology Centre (since 2006) - Genetics ; Molecular Biology R&D Projects GA19-20697S GA ČR - Czech Science Foundation (CSF) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UPT-D - RVO:68081731 ; BC-A - RVO:60077344 UT WOS 000795917600010 EID SCOPUS 85126842499 DOI 10.1016/j.ijbiomac.2022.03.102 Annotation Polyhydroxyalkanoates (PHA) are abundant microbial polyesters accumulated in the form of intracellular granules by numerous prokaryotes primarily as storage of carbon and energy. Apart from their storage function, the presence of PHA also enhances the robustness of the microbial cells against various stressors. In this work, we investigated the role of PHA in Cupriavidus necator, a model organism concerning PHA metabolism, for adaptation to osmotic pressure and copper ions. In long-term laboratory evolution experiments, the bacterial culture was cultivated in presence of elevated doses of sodium chloride or copper ions (incubations lasted 78 passages for Cu2+ and 68 passages for NaCl) and the evolved strains were compared with the wild-type strain in terms of growth and PHA production capacity, cell morphology (investigated by various electron microscopy techniques), activities of selected enzymes involved in PHA metabolism and other crucial metabolic pathways, the chemical composition of bacterial biomass (determined by infrared and Raman spectroscopy) and also considering robustness against various stressors. The results confirmed the important role of PHA metabolism for adaptation to both tested stressors. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2023 Electronic address https://www.sciencedirect.com/science/article/pii/S0141813022005712
Number of the records: 1