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Chronopotentiometric sensing of native, oligomeric, denatured and aggregated serum albumin at charged surfaces
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SYSNO ASEP 0565321 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chronopotentiometric sensing of native, oligomeric, denatured and aggregated serum albumin at charged surfaces Author(s) Římánková, Ludmila (BFU-R)
Černocká, Hana (BFU-R) RID, ORCID
Tihlaříková, Eva (UPT-D) RID, ORCID, SAI
Neděla, Vilém (UPT-D) RID, ORCID, SAI
Ostatná, Veronika (BFU-R) RID, ORCIDNumber of authors 5 Article number 108100 Source Title Bioelectrochemistry. - : Elsevier - ISSN 1567-5394
Roč. 145, JUN 2022 (2022)Number of pages 6 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords Constant current chronopotentiometry ; Serum albumin ; Freezing ; Electrode ; Charged surface ; Scanning transmission electron microscopy Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology Subject RIV - cooperation Institute of Scientific Instruments - Biochemistry R&D Projects GA18-18154S GA ČR - Czech Science Foundation (CSF) GA19-08239S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 ; UPT-D - RVO:68081731 UT WOS 000790444700005 EID SCOPUS 85126671200 DOI 10.1016/j.bioelechem.2022.108100 Annotation In protein analysis, fast techniques applicable for preliminary tests of the protein structural changes are sought. We show that using constant current chronopotentiometric stripping peak H, small amounts of oligomeric, denatured and aggregated bovine serum albumin (BSA) can be easily distinguished from native form. Different behavior of native, denatured, and aggregated BSA could be explained by combination of their different adsorption at charged surface and accessibility of electroactive amino acid residues. Ability to discriminate between individual forms allows to use chronopotentiometric stripping for study of processes responsible for structural changes, such as freezing treatment. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2023 Electronic address https://reader.elsevier.com/reader/sd/pii/S1567539422000512?token=2776BB9FAD61EA7FFA4A4907416959A460AF8BD9CF30AEA52A04012B6503D97AEAB245621A71F06366F7B538647E27C5&originRegion=eu-west-1&originCreation=20221212135323
Number of the records: 1