Traceless enzymatic synthesis of monodispersed hypermodified oligodeoxyribonucleotide polymers from RNA templates

0561972 - ÚOCHB 2023 RIV GB eng J - Journal Article
Ondruš, Marek - Sýkorová, Veronika - Hocek, Michal
Traceless enzymatic synthesis of monodispersed hypermodified oligodeoxyribonucleotide polymers from RNA templates.
Chemical Communications. Roč. 58, č. 80 (2022), s. 11248-11251. ISSN 1359-7345. E-ISSN 1364-548X
R&D Projects: GA ČR(CZ) GX20-00885X
Institutional support: RVO:61388963
Keywords : exponential enrichment * reverse transcriptase * systematic evolution
OECD category: Biochemistry and molecular biology
Impact factor: 4.9, year: 2022
Method of publishing: Limited access
https://doi.org/10.1039/D2CC03588J

We have developed a new alternative for enzymatic synthesis of single-stranded hypermodified oligodeoxyribonucleotides displaying four different hydrophobic groups based on reverse transcription from RNA templates catalyzed by DNA polymerases using a set of base-modified dNTPs followed by digestion of RNA by RNases. Using mixed oligodeoxyribonucleotide primers containing a ribonucleotide at the 3'-end, RNase AT1 simultaneously digested the template and cleaved off the primer to release a fully modified oligonucleotide that can be further 3'-labelled with a fluorescent nucleotide using TdT. The resulting hypermodified oligonucleotides could find applications in selection of aptamers or other functional macromolecules.
Permanent Link: https://hdl.handle.net/11104/0334407