Isolation and Characterization of Two Lytic Phages Efficient Against Phytopathogenic Bacteria From Pseudomonas and Xanthomonas Genera

0561305 - ÚEB 2023 RIV CH eng J - Journal Article
Korniienko, Nataliia - Kharina, A. - Zrelovs, N. - Jindřichová, Barbora - Moravec, Tomáš - Budzanivska, I. - Burketová, Lenka - Kalachova, Tetiana
Isolation and Characterization of Two Lytic Phages Efficient Against Phytopathogenic Bacteria From Pseudomonas and Xanthomonas Genera.
Frontiers in Microbiology. Roč. 13, APR 25 (2022), č. článku 853593. ISSN 1664-302X. E-ISSN 1664-302X
R&D Projects: GA MŠMT(CZ) EF16_019/0000738; GA MŠMT(CZ) LM2018129
Institutional support: RVO:61389030
Keywords : Autographiviridae * bacteriophage (phage) * biocontrol * phytopathogenic bacteria * Pseudomonas syringae pv. tomato
OECD category: Biochemical research methods
Impact factor: 5.2, year: 2022
Method of publishing: Open access
https://doi.org/10.3389/fmicb.2022.853593

Pseudomonas syringae is a bacterial pathogen that causes yield losses in various economically important plant species. At the same time, P. syringae pv. tomato (Pst) is one of the best-studied bacterial phytopathogens and a popular model organism. In this study, we report on the isolation of two phages from the market-bought pepper fruit showing symptoms of bacterial speck. These Pseudomonas phages were named Eir4 and Eisa9 and characterized using traditional microbiological methods and whole-genome sequencing followed by various bioinformatics approaches. Both of the isolated phages were capable only of the lytic life cycle and were efficient against several pathovars from Pseudomonas and Xanthomonas genera. With the combination of transmission electron microscopy (TEM) virion morphology inspection and comparative genomics analyses, both of the phages were classified as members of the Autographiviridae family with different degrees of novelty within the known phage diversity. Eir4, but not Eisa9, phage application significantly decreased the propagation of Pst in the leaf tissues of Arabidopsis thaliana plants. The biological properties of Eir4 phage allow us to propose it as a potential biocontrol agent for use in the prevention of Pst-associated bacterioses and also as a model organism for the future research of mechanisms of phage–host interactions in different plant systems.
Permanent Link: https://hdl.handle.net/11104/0333994