Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis

0560366 - ÚEB 2023 RIV CH eng J - Journal Article
Ivanizs, L. - Marcotuli, I. - Rakszegi, M. - Kalapos, B. - Szőke-Pázsi, K. - Farkas, A. - Türkösi, E. - Gaál, E. - Kruppa, K. - Kovács, P. - Darkó, É. - Szakács, É. - Said, Mahmoud - Cápal, Petr - Doležel, Jaroslav - Gadaleta, A. - Molnár, I.
Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis.
International Journal of Molecular Sciences. Roč. 23, č. 7 (2022), č. článku 3821. E-ISSN 1422-0067
R&D Projects: GA MŠMT(CZ) EF16_019/0000827; GA MŠMT(CZ) LM2015047
Institutional support: RVO:61389030
Keywords : Aegilops biuncialis * DArTseq analysis * dietary fiber * genome-wide association study (GWAS) * β-glucan
OECD category: Biochemistry and molecular biology
Impact factor: 5.6, year: 2022
Method of publishing: Open access
https://doi.org/10.3390/ijms23073821

Grain dietary fiber content is an important health-promoting trait of bread wheat. A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition. In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae. biuncialis. A large variation in grain protein and edible fiber content was revealed, reflecting the origin of Ae. biuncialis accessions from different eco-geographical habitats. Association analysis using DArTseq-derived SNPs identified 34 QTLs associated with β-glucan, pentosan, water-extractable pentosan, and protein content. Mapping the markers to draft chromosome assemblies of diploid progenitors of Ae. biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total-and water-extractable pentosan content. Functional annotation of the associated marker sequences identified fourteen genes, nine of which were identified in other monocots. The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
Permanent Link: https://hdl.handle.net/11104/0333299