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Visualization of Sentinel Lymph Nodes with Mannosylated Fluorescent Nanodiamonds
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SYSNO ASEP 0556784 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Visualization of Sentinel Lymph Nodes with Mannosylated Fluorescent Nanodiamonds Author(s) Kvaková, Klaudia (UOCHB-X) ORCID
Ondra, M. (CZ)
Schimer, Jiří (UOCHB-X) RID, ORCID
Petrík, M. (CZ)
Nový, Z. (CZ)
Raabová, Helena (UOCHB-X)
Hajdúch, M. (CZ)
Cígler, Petr (UOCHB-X) RID, ORCIDArticle number 2109960 Source Title Advanced Functional Materials - ISSN 1616-301X
Roč. 32, č. 23 (2022)Number of pages 14 s. Language eng - English Country DE - Germany Keywords cancer imaging ; fluorescent nanodiamonds ; lymph nodes ; macrophages ; mannose OECD category Radiology, nuclear medicine and medical imaging R&D Projects EF16_026/0008382 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA18-17071S GA ČR - Czech Science Foundation (CSF) LM2018133 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UOCHB-X - RVO:61388963 UT WOS 000763337400001 EID SCOPUS 85125586919 DOI 10.1002/adfm.202109960 Annotation The present study focuses on the development of a sensitive and specific approach for the visualization of sentinel lymph nodes draining the tumor using ultrabright 200-nm fluorescent nanodiamonds (FNDs) equipped with a designed targeting surface architecture. The FNDs with a narrow size distribution are isolated by differential centrifugation, colloidally stabilized with alkyne-functionalized poly(glycerol) and modified with a polyvalent array of mannose (FND-p-Man). In vitro experiments demonstrate an outstanding increase of the particle internalization by the mannose receptor CD206 (MR) and no significant toxicity. MR involvement is confirmed by blocking ligand binding with mannan and a 15.2 mAb (specific anti-MR) in J774A.1 mouse macrophage cell line. In vivo mouse experiments confirm increased retention of FND-p-Man in sentinel lymph nodes of both healthy and B16 melanoma bearing animals. These results suggest that FND-p-Man has potential as a tracer for lymph node visualization in locoregional perioperative cancer diagnostics and as a tool for endoscopic/robotic fluorescence-guided surgery. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2023 Electronic address https://doi.org/10.1002/adfm.202109960
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