Fast Fluoroalkylation of Proteins Uncovers the Structure and Dynamics of Biological Macromolecules

0556422 - BTÚ 2022 RIV US eng J - Journal Article
Fojtik, L. - Fiala, J. - Pompach, Petr - Chmelík, J. - Matoušek, V. - Beier, P. - Kukačka, Z. - Novák, P.
Fast Fluoroalkylation of Proteins Uncovers the Structure and Dynamics of Biological Macromolecules.
Journal of the American Chemical Society. Roč. 143, č. 49 (2021), s. 20670-20679. ISSN 0002-7863. E-ISSN 1520-5126
Institutional support: RVO:86652036
Keywords : fast photochemical oxidation * hypervalent iodine reagents * mass-spectrometry * top-down * myoglobin
OECD category: Analytical chemistry
Impact factor: 16.383, year: 2021
Method of publishing: Open access
https://pubs.acs.org/doi/10.1021/jacs.1c07771

Covalent labeling of proteins in combination with mass spectrometry has been established as a complementary technique to classical structural methods, such as X-ray, NMR, or cryogenic electron microscopy (Cryo-EM), used for protein structure determination. Although the current covalent labeling techniques enable the protein solvent accessible areas with sufficient spatial resolution to be monitored, there is still high demand for alternative, less complicated, and inexpensive approaches. Here, we introduce a new covalent labeling method based on fast fluoroalkylation of proteins (FFAP). FFAP uses fluoroalkyl radicals formed by reductive decomposition of Togni reagents with ascorbic acid to label proteins on a time scale of seconds. The feasibility of FFAP to effectively label proteins was demonstrated by monitoring the differential amino acids modification of native horse heart apomyoglobin/holomyoglobin and the human haptoglobin-hemoglobin complex. The obtained data confirmed the Togni reagent-mediated FFAP is an advantageous alternative method for covalent labeling in applications such as protein footprinting and epitope mapping of proteins (and their complexes) in general. Data are accessible via the ProteomeXchange server with the data set identifier PXD027310.
Permanent Link: http://hdl.handle.net/11104/0330641