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Transcriptome Response to Cadmium Exposure in Barley (Hordeum vulgare L.)
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SYSNO ASEP 0554848 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptome Response to Cadmium Exposure in Barley (Hordeum vulgare L.) Author(s) Kintlova, M. (CZ)
Vrána, J. (CZ)
Hobza, Roman (BFU-R) RID, ORCID
Blavet, Nicolas (BFU-R) ORCID
Hudzieczek, Vojtěch (BFU-R) ORCIDNumber of authors 5 Article number 629089 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 12, JUL 15 2021 (2021)Number of pages 13 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords metal-ion transporter ; expression differences ; arabidopsis-thaliana ; zip transporters ; zn-deficiency ; cell-cycle ; genes Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Open access Institutional support BFU-R - RVO:68081707 UT WOS 000679137400001 EID SCOPUS 85111569464 DOI 10.3389/fpls.2021.629089 Annotation Cadmium is an environmental pollutant with high toxicity that negatively affects plant growth and development. To understand the molecular mechanisms of plant response to cadmium stress, we have performed a genome-wide transcriptome analysis on barley plants treated with an increased concentration of cadmium. Differential gene expression analysis revealed 10,282 deregulated transcripts present in the roots and 7,104 in the shoots. Among them, we identified genes related to reactive oxygen species metabolism, cell wall formation and maintenance, ion membrane transport and stress response. One of the most upregulated genes was PLANT CADMIUM RESISTACE 2 (HvPCR2) known to be responsible for heavy metal detoxification in plants. Surprisingly, in the transcriptomic data we identified four different copies of the HvPCR2 gene with a specific pattern of upregulation in individual tissues. Heterologous expression of all five barley copies in a Cd-sensitive yeast mutant restored cadmium resistance. In addition, four HvPCR2 were located in tandem arrangement in a single genomic region of the barley 5H chromosome. To our knowledge, this is the first example showing multiplication of the PCR2 gene in plants. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2022 Electronic address https://www.frontiersin.org/articles/10.3389/fpls.2021.629089/full
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