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Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus
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SYSNO ASEP 0553502 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Trichloromethane dechlorination by a novel Dehalobacter sp. strain 8M reveals a third contrasting C and Cl isotope fractionation pattern within this genus Author(s) Soder-Walz, J. (ES)
Torrento, C. (ES)
Algora, Camelia (MBU-M) ORCID
Wasmund, K. (AT)
Cortes, P. (ES)
Soler, A. (ES)
Vicent, T. (ES)
Rosell, M. (ES)
Marco-Urrea, E. (ES)Article number 152659 Source Title Science of the Total Environment. - : Elsevier - ISSN 0048-9697
Roč. 813, MAR 20 2022 (2022)Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords microbial reductive dehalogenation ; enrichment culture ; chloroform ; dichloromethane ; carbon ; tetrachloroethene ; respiration ; degradation ; Trichloromethane ; 1,1,2-trichloroethane ; Dehalobacter ; Organohalide respiration ; Isotopic fractionation ; 2d-csia Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000748713900012 EID SCOPUS 85121909144 DOI 10.1016/j.scitotenv.2021.152659 Annotation Trichloromethane (TCM) is a pollutant frequently detected in contaminated aquifers, and only four bacterial strains are known to respire it. Here, we obtained a novel Dehalobacter strain capable of transforming TCM to dichloromethane, which was denominated Dehalobacter sp. strain 8M. Besides TCM, strain 8M also completely transformed 1,1,2-trichloroethane to vinyl chloride and 1,2-dichloroethane. Quantitative PCR analysis for the 16S rRNA genes confirmed growth of Dehalobacter with TCM and 1,1,2-trichloroethane as electron acceptors. Carbon and chlorine isotope fractionation during TCM transformation was studied in cultured cells and in enzymatic assays with cell suspensions and crude protein extracts. TCM transformation in the three studied systems resulted in small but significant carbon (epsilon(C) =2.7 +/- 0.1 parts per thousand for respiring cells,3.1 +/- 0.1 parts per thousand for cell suspensions, and 4.1 +/- 0.5 parts per thousand for crude protein extracts) and chlorine (epsilon(Cl) =0.9 +/- 0.1 parts per thousand,1.1 +/- 0.1 parts per thousand, and 1.2 +/- 0.2 parts per thousand, respectively) isotope fractionation. A characteristic and consistent dual CCl isotope fractionation pattern was observed for the three systems (combined Lambda(C/Cl) = 2.8 +/- 0.3). This Lambda(C/Cl) differed significantly from previously reported values for anaerobic dechlorination of TCM by the corrinoid cofactor vitamin B12 and other Dehalobacter strains. These findings widen our knowledge on the existence of different enzyme binding mechanisms underlying TCM-dechlorination within the genus Dehalobacter and demonstrates that dual isotope analysis could be a feasible tool to differentiate TCM degraders at field studies. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.sciencedirect.com/science/article/pii/S0048969721077378?via%3Dihub
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