Conplastic strains for identification of retrograde effects of mitochondrial dna variation on cardiometabolic traits in the spontaneously hypertensive rat

Pravenec, Michal; Šilhavý, Jan; Mlejnek, Petr; Šimáková, Miroslava; Mráček, Tomáš; Pecinová, Alena; Tauchmannová, Kateřina; Hüttl, M.; Malínská, H.; Kazdová, L.; Neckář, Jan; Kolář, František; Žurmanová, J.; Novotný, J.; Houštěk, Josef

doi:https://dx.doi.org/10.33549/physiolres.934740

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Conplastic strains for identification of retrograde effects of mitochondrial dna variation on cardiometabolic traits in the spontaneously hypertensive rat

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SYSNO ASEP	0553221
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Conplastic strains for identification of retrograde effects of mitochondrial dna variation on cardiometabolic traits in the spontaneously hypertensive rat
Author(s)	Pravenec, Michal (FGU-C)_{RID, ORCID} Šilhavý, Jan (FGU-C)_{RID, ORCID} Mlejnek, Petr (FGU-C)_{RID, ORCID} Šimáková, Miroslava (FGU-C)_{RID, ORCID} Mráček, Tomáš (FGU-C)_{RID, ORCID} Pecinová, Alena (FGU-C)_{RID, ORCID, SAI} Tauchmannová, Kateřina (FGU-C)_{RID, ORCID} Hüttl, M. (CZ) Malínská, H. (CZ) Kazdová, L. (CZ) Neckář, Jan (FGU-C)_{RID, ORCID} Kolář, František (FGU-C)_{RID, ORCID, SAI} Žurmanová, J. (CZ) Novotný, J. (CZ) Houštěk, Josef (FGU-C)_{RID, ORCID}
Source Title	Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408 Roč. 70, Suppl.4 (2021), S471-S481
Number of pages	11 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	mitochondrial DNA ; conplastic strains ; spontaneously hypertensive rat ; glucose and lipid metabolism ; cardiac traits
OECD category	Physiology (including cytology)
R&D Projects	GA19-10354S GA ČR - Czech Science Foundation (CSF)
	GA18-03207S GA ČR - Czech Science Foundation (CSF)
	GA17-07748S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	FGU-C - RVO:67985823
UT WOS	000768842600003
EID SCOPUS	85125098524
DOI	10.33549/physiolres.934740
Annotation	Mitochondrial retrograde signaling is a pathway of communication from mitochondria to the nucleus. Recently, natural mitochondrial genome (mtDNA) polymorphisms (haplogroups) received increasing attention in the pathophysiology of human common diseases. However, retrograde effects of mtDNA variants on such traits are difficult to study in humans. The conplastic strains represent key animal models to elucidate regulatory roles of mtDNA haplogroups on defined nuclear genome background. To analyze the relationship between mtDNA variants and cardiometabolic traits, we derived a set of rat conplastic strains (SHR-mtBN, SHR-mtF344 and SHR-mtLEW), harboring all major mtDNA haplotypes present in common inbred strains on the nuclear background of the spontaneously hypertensive rat (SHR). The BN, F344 and LEW mtDNA differ from the SHR in multiple amino acid substitutions in protein coding genes and also in variants of tRNA and rRNA genes. Different mtDNA haplotypes were found to predispose to various sets of cardiometabolic phenotypes which provided evidence for significant retrograde effects of mtDNA in the SHR. In the future, these animals could be used to decipher individual biochemical components involved in the retrograde signaling.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2022
Electronic address	https://www.biomed.cas.cz/physiolres/pdf/2021/70_S471.pdf

Number of the records: 1