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Mass Spectrometry Imaging of Small Molecules: Methods and Protocols
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SYSNO ASEP 0551179 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title MALDI Mass Spectrometry Imaging of Lipids on Free-Floating Brain Sections and Immunohistochemically Colocalized Markers of Neurodegeneration Author(s) Strnad, Štěpán (UOCHB-X) ORCID
Strnadová, Veronika (UOCHB-X) ORCID
Sýkora, D. (CZ)
Cvačka, Josef (UOCHB-X) RID, ORCID
Maletínská, Lenka (UOCHB-X) RID, ORCID
Vrkoslav, Vladimír (UOCHB-X) RID, ORCIDSource Title Mass Spectrometry Imaging of Small Molecules: Methods and Protocols. - New York : Humana Press, 2022 / Lee Y.-J. - ISSN 1064-3745 - ISBN 978-1-07162029-8 Pages s. 229-239 Number of pages 11 s. Number of pages 275 Publication form Print - P Language eng - English Country US - United States Keywords colocalization ; free-floating ; immunohistochemistry ; MALDI MSI ; multimodal ; tissue section OECD category Physiology (including cytology) R&D Projects GA20-00546S GA ČR - Czech Science Foundation (CSF) Institutional support UOCHB-X - RVO:61388963 EID SCOPUS 85121534724 DOI https://doi.org/10.1007/978-1-0716-2030-4_16 Annotation In mass spectrometry imaging (MSI), the essential steps in sample preparation include collection and storage. The most widely used preservation procedure for MSI consists in freezing samples and storing them at temperatures below −80 °C. On the other hand, the most common method for preserving biological samples in clinical practice is their fixation in paraformaldehyde. The storage of free-floating sections is a particular type of the preservation of paraformaldehyde-fixed tissues that is used in immunohistochemistry. This chapter describes the approach of the multimodal imaging of free-floating brain sections using the MSI of lipids and the immunohistochemistry of neurodegeneration markers. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2023 Electronic address https://doi.org/10.1007/978-1-0716-2030-4_16
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