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Myomedin scaffold variants targeted to 10E8 HIV-1 broadly neutralizing antibody mimic gp41 epitope and elicit HIV-1 virus-neutralizing sera in mice
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SYSNO ASEP 0549194 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Myomedin scaffold variants targeted to 10E8 HIV-1 broadly neutralizing antibody mimic gp41 epitope and elicit HIV-1 virus-neutralizing sera in mice Author(s) Kuchař, Milan (BTO-N) RID
Kosztyu, P. (CZ)
Daniel Lišková, Veronika (BTO-N)
Černý, Jiří (BTO-N) RID, ORCID
Petroková, Hana (BTO-N) RID
Vroblova, E. (CZ)
Malý, Michal (BTO-N)
Vaňková, Lucie (BTO-N)
Krupka, M. (CZ)
Raskova Kafkova, L. (CZ)
Turánek Knotigová, P. (CZ)
Dušková, Jarmila (BTO-N) RID, SAI
Dohnálek, Jan (BTO-N) RID, ORCID
Mašek, J. (CZ)
Turánek, J. (CZ)
Raška, M. (CZ)
Malý, Petr (BTO-N) RID, ORCIDNumber of authors 17 Source Title Virulence. - : Taylor & Francis - ISSN 2150-5594
Roč. 12, č. 1 (2021), s. 1271-1287Number of pages 17 s. Language eng - English Country US - United States Keywords protein scaffold ; combinatorial library ; protein mimetics ; Env glycoprotein Subject RIV FN - Epidemiology, Contagious Diseases ; Clinical Immunology OECD category Infectious Diseases R&D Projects NV15-32198A GA MZd - Ministry of Health (MZ) EF16_025/0007397 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BTO-N - RVO:86652036 UT WOS 000650967900001 EID SCOPUS 85105945438 DOI 10.1080/21505594.2021.1920251 Annotation One of the proposed strategies for the development of a more efficient HIV-1 vaccine is based on the identification of proteins binding to a paratope of chosen broadly neutralizing antibody (bNAb) that will mimic cognate HIV-1 Env (glyco)protein epitope and could be used as potent immunogens for induction of protective virus-neutralizing antibodies in the immunized individuals. To verify this non-cognate ligand concept, we developed a highly complex combinatorial library designed on a scaffold of human myomesin-1 protein domain and selected proteins called Myomedins specifically binding to variable regions of HIV-1 broadly neutralizing antibody 10E8. Immunization of mice with these Myomedin variants elicited the production of HIV-1 Env-specific antibodies. Hyperimmune sera bound to Env pseudotyped viruses and weakly/moderately neutralized 54% of tested clade A, B, C, and AE pseudotyped viruses variants in vitro. These results demonstrate that Myomedin variants have the potential to mimic Env epitopes and could be used as potential HIV-1 vaccine components. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2022 Electronic address https://www.tandfonline.com/doi/full/10.1080/21505594.2021.1920251
Number of the records: 1