Number of the records: 1
Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica
- 1.
SYSNO ASEP 0546812 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica Author(s) Miláček, Matěj (BC-A) ORCID
Bittová, Lenka (BC-A) RID, ORCID
Tůmová, Šárka (BC-A) ORCID
Lukšan, Ondřej (UOCHB-X)
Hanus, Robert (UOCHB-X) RID, ORCID
Kyjaková, Pavlína (UOCHB-X) RID
Machara, Aleš (UOCHB-X) ORCID
Marek, Aleš (UOCHB-X) RID, ORCID
Jindra, Marek (BC-A) RID, ORCIDNumber of authors 9 Article number 103671 Source Title Insect Biochemistry and Molecular Biology. - : Elsevier - ISSN 0965-1748
Roč. 139, DEC 01 (2021)Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords juvenile hormone ; methoprene-tolerant ; hormone receptor Subject RIV ED - Physiology OECD category Biochemistry and molecular biology R&D Projects GX20-05151X GA ČR - Czech Science Foundation (CSF) LM2015064 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018131 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BC-A - RVO:60077344 ; UOCHB-X - RVO:61388963 UT WOS 000711780700006 EID SCOPUS 85117203283 DOI 10.1016/j.ibmb.2021.103671 Annotation Juvenile hormone (JH) controls insect reproduction and development through an intracellular receptor complex comprising two bHLH-PAS proteins, the JH-binding Methoprene-tolerant (Met) and its partner Taiman (Tai). Many hemimetabolous insects including cockroaches strictly depend on JH for stimulation of vitellogenesis. In termites, the eusocial hemimetabolans, JH also regulates the development of caste polyphenism. Studies addressing the agonist ligand binding to recombinant JH receptors currently include three species belonging to two holometabolous insect orders, but none that would represent any of the hemimetabolous orders. Here, we examined JH receptors in two representatives of Blattodea, the cockroach Blattella germanica and the termite Prorhinotermes simplex. To test the JH-binding capacity of Met proteins from these species, we performed chemical synthesis and tritium labeling of the natural blattodean JH homolog, JH III. Our improved protocol increased the yield and specific activity of [10-3H]JH III relative to formerly available preparations. Met proteins from both species specifically bound [3H]JH III with high affinity, whereas Met variants mutated at a critical position within the ligand-binding domain were incapable of such binding. Furthermore, JH III and the synthetic JH mimic fenoxycarb stimulated dimerization between Met and Tai components of the respective JH receptors of both species. These data present primary evidence for agonist binding by JH receptors in any hemimetabolous species and provide a molecular basis for JH action in cockroaches and termites. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0965174821001545?via%3Dihub
Number of the records: 1