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Molecular and subcellular cryoinjury of fish spermatozoa and approaches to improve cryopreservation
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SYSNO ASEP 0541307 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Molecular and subcellular cryoinjury of fish spermatozoa and approaches to improve cryopreservation Author(s) Xin, M.M. (CZ)
Niksirat, H. (CZ)
Shaliutina-Kolešová, A. (CZ)
Siddique, M.A.M. (BD)
Štěrba, Ján (BC-A) RID, ORCID
Boryshpolets, S. (CZ)
Linhart, O. (CZ)Number of authors 7 Source Title Reviews in Aquaculture. - : Wiley - ISSN 1753-5123
Roč. 12, č. 2 (2020), s. 909-924Number of pages 16 s. Publication form Print - P Language eng - English Country US - United States Keywords seminal plasma-proteins ; carp cyprinus-carpio ; trout oncorhynchus-mykiss ; sperm dna-damage ; antifreeze proteins ; oxidative stress ; fertilization capacity ; spontaneous activation ; lipid-peroxidation ; proteomic analysis ; antifreeze glycoproteins ; antifreeze proteins ; antioxidants ; cryodamage ; seminal plasma ; spermatozoa Subject RIV EI - Biotechnology ; Bionics OECD category Industrial biotechnology R&D Projects EE2.3.30.0032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BC-A - RVO:60077344 UT WOS 000531069200019 EID SCOPUS 85067402725 DOI 10.1111/raq.12355 Annotation The quality of frozen/thawed fish sperm is generally lower than that of fresh sperm. Extremely low temperatures are associated with damage to fish spermatozoon subcellular compartments and associated molecules such as DNA and proteins. Cryodamage can negatively affect DNA integrity, spermatozoon metabolism and motility, consequently impairing fertilization and embryo development. To preserve sperm efficiently, the addition of proteins including seminal plasma proteins, antioxidants, antifreeze proteins and antifreeze glycoproteins, and bovine serum albumin before and/or after freezing is suggested to minimize cryoinjury. An appropriate quantity of seminal plasma components in cryopreservation medium can prevent spermatozoon membrane damage by maintaining antioxidant enzymes and their proper distribution on the spermatozoon surface. Under storage conditions, the semen antioxidant system is ineffective in protecting spermatozoa from reactive oxygen species. The addition of antioxidants can provide protection for spermatozoa and reduce cryoinjuries during storage, but the effect of a given antioxidant is species-specific. Antifreeze proteins and antifreeze glycoproteins can stabilize the cell membrane via interaction with phospholipid components and inhibit ice-crystal growth to reduce damage related to osmotic stress and ice crystallization. However, they may exhibit a cytotoxic effect. This review summarizes the sources and characteristics of subcellular spermatozoon cryoinjury and discusses approaches to improve outcome of fish sperm cryopreservation. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://onlinelibrary.wiley.com/doi/epdf/10.1111/raq.12355
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