Number of the records: 1
Colloidally stable P(DMA-AGME)-Ale-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles as a multimodal contrast agent for down- and upconversion luminescence, magnetic resonance imaging, and computed tomography
- 1.
SYSNO ASEP 0537739 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Colloidally stable P(DMA-AGME)-Ale-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles as a multimodal contrast agent for down- and upconversion luminescence, magnetic resonance imaging, and computed tomography Author(s) Shapoval, Oleksandr (UMCH-V) ORCID, RID
Oleksa, Viktoriia (UMCH-V) RID
Šlouf, Miroslav (UMCH-V) RID, ORCID
Lobaz, Volodymyr (UMCH-V) RID, ORCID
Trhlíková, Olga (UMCH-V) RID, ORCID
Filipová, Marcela (UMCH-V) RID, ORCID
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Engstová, Hana (FGU-C) RID, ORCID
Pankrác, J. (CZ)
Modrý, A. (CZ)
Herynek, V. (CZ)
Ježek, Petr (FGU-C) RID, ORCID
Šefc, L. (CZ)
Horák, Daniel (UMCH-V) RID, ORCIDArticle number 230 Source Title Nanomaterials. - : MDPI - ISSN 2079-4991
Roč. 11, č. 1 (2021)Number of pages 21 s. Language eng - English Country CH - Switzerland Keywords up-conversion luminescence ; down-conversion luminescence ; colloidal stability Subject RIV CD - Macromolecular Chemistry OECD category Polymer science Subject RIV - cooperation Institute of Physiology - Cell Biology R&D Projects GA19-00676S GA ČR - Czech Science Foundation (CSF) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC19032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823 UT WOS 000610663900001 EID SCOPUS 85100140596 DOI https://doi.org/10.3390/nano11010230 Annotation Multimodal imaging, integrating several modalities including down- and up-conversion luminescence, T1- and T2(T2*)-weighted MRI, and CT contrasting in one system, is very promising for improved diagnosis of severe medical disorders. To reach the goal, it is necessary to develop suitable nanoparticles that are highly colloidally stable in biologically relevant media. Here, hydrophilic poly(N,N-dimethylacrylamide-N-acryloylglycine methyl ester)-alendronate-[P(DMA-AGME)-Ale]-coated Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ nanoparticles were synthesized by a coprecipitation method in ethylene glycol (EG) followed by coating with the polymer. The particles were tho-roughly characterized by a dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray energy dispersive spectroscopy (EDAX), selected area electron diffraction (SAED), elemental ana-lysis and fluorescence spectroscopy. Aqueous particle dispersions exhibited excellent colloidal stability in water and physiological buffers. In vitro toxicity assessments suggested no or only mild toxicity of the surface-engineered Gd(Tb)F3:Tb3+(Gd3+),Yb3+,Nd3+ particles in a wide range of concentrations. Internalization of the particles by several types of cells, including HeLa, HF, HepG2, and INS, was confirmed by a down- and up-conversion confocal microscopy. Newly developed particles thus proved to be an efficient contrast agent for fluorescence imaging, T1- and T2(T2*)-weighted magnetic resonance imaging (MRI), and computed tomography (CT). Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2022 Electronic address https://www.mdpi.com/2079-4991/11/1/230
Number of the records: 1