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Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments
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SYSNO ASEP 0537134 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments Author(s) Fuente Herraiz, David (UEK-B)
Lazár, D. (CZ)
Oliver-Villanueva, J. V. (ES)
Urchueguía, J. F. (ES)Number of authors 4 Source Title Photosynthesis Research. - : Springer - ISSN 0166-8595
Roč. 147, č. 1 (2021), s. 75-90Number of pages 15 s. Language eng - English Country NL - Netherlands Keywords photosystem-i ; wavelength dependence ; state transitions ; light-absorption ; chlorophyll-a ; sp pcc-6803 ; carotenoids ; phytoplankton ; growth ; myxoxanthophyll ; Absorption ; Spectrum ; Light ; Pigment ; Modeling ; Synechocystis ; Photosystem Subject RIV BO - Biophysics OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology Research Infrastructure CzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i. Method of publishing Limited access Institutional support UEK-B - RVO:86652079 UT WOS 000593428700001 EID SCOPUS 85096880710 DOI 10.1007/s11120-020-00799-8 Annotation In this work, we reconstructed the absorption spectrum of different Synechocystis sp. PCC 6803 optical strains by summing the computed signature of all pigments present in this organism. To do so, modifications to in vitro pigment spectra were first required: namely wavelength shift, curve smoothing, and the package effect calculation derived from high pigment densities were applied. As a result, we outlined a plausible shape for the in vivo absorption spectrum of each chromophore. These are flatter and slightly broader in physiological conditions yet the mean weight-specific absorption coefficient remains identical to the in vitro conditions. Moreover, we give an estimate of all pigment concentrations without applying spectrophotometric correlations, which are often prone to error. The computed cell spectrum reproduces in an accurate manner the experimental spectrum for all the studied wavelengths in the wild-type, Olive, and PAL strain. The gathered pigment concentrations are in agreement with reported values in literature. Moreover, different illumination set-ups were evaluated to calculate the mean absorption cross-section of each chromophore. Finally, a qualitative estimate of light-limited cellular growth at each wavelength is given. This investigation describes a novel way to approach the cell absorption spectrum and shows all its inherent potential for photosynthesis research. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2022 Electronic address https://link.springer.com/article/10.1007/s11120-020-00799-8
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