Translational Research in Serious Human Diseases

0536527 - ÚŽFG 2021 RIV CZ eng M - Monography Chapter
Tylečková, Jiřina - Červenka, Jakub - Vodička, Petr
Targeted proteomics in translational research of neurodegenerative diseases.
Translational Research in Serious Human Diseases. Praha: Academia, 2020 - (Kello, M.; Strnadel, J.; Klempir, J.; Roth, J.; Myslivcová-Fučíková, A.; Hansíková, H.; Kozák, I.), s. 112-122. ISBN 978-80-200-3158-7
R&D Projects: GA MŠMT(CZ) LO1609
Institutional support: RVO:67985904
Keywords : proteomics * targeted mass spectrometry * selected reaction monitoring
OECD category: Biochemistry and molecular biology

With recent technical improvements in the field of mass spectrometry-based analysis of proteins, the technology is now ready to enter clinical laboratories for routine measurements of protein biomarkers. Targeted MS methods like selected reaction monitoring or recently presented parallel reaction monitoring showed a great potential for sensitive, selective, and robust assays. Ease of development and multiplexing capacity of these methods is especially suitable for verification and validation of proteomics discovery data. The translation of preclinical findings in research of neurodegenerative disorders into practical use now relies on necessary implementation of independent tightly controlled clinical studies on a large cohort of well-defined subjects. In our review we briefly describe the targeted proteomics techniques and their employment in clinical proteomics validation of neurodegeneration biomarkers, with hope to identify disease diagnostic, prognostic or predictive biomarkers.
Permanent Link: http://hdl.handle.net/11104/0314299