Number of the records: 1
Age-related differences in the translational landscape of mammalian oocytes
- 1.
SYSNO ASEP 0536356 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Age-related differences in the translational landscape of mammalian oocytes Author(s) del Llano, Edgar (UZFG-Y) ORCID
Mašek, T. (CZ)
Gahurová, Lenka (UZFG-Y) ORCID
Pospíšek, M. (CZ)
Končická, Markéta (UZFG-Y) ORCID
Jindrová, Anna (UZFG-Y) ORCID
Jansová, Denisa (UZFG-Y) ORCID
Iyyappan, Rajan (UZFG-Y) ORCID
Roučová, K. (CZ)
Bruce, A. W. (CZ)
Kubelka, Michal (UZFG-Y) RID, ORCID
Šušor, Andrej (UZFG-Y) RID, ORCIDArticle number e13231 Source Title Aging Cell. - : Wiley - ISSN 1474-9718
Roč. 19, č. 10 (2020)Number of pages 13 s. Publication form Online - E Language eng - English Country US - United States Keywords aging ; cytokinesis ; meiosis ; oocyte ; translation Subject RIV EB - Genetics ; Molecular Biology OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology R&D Projects GA19-13491S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 000570994100001 EID SCOPUS 85091109400 DOI 10.1111/acel.13231 Annotation Increasing maternal age in mammals is associated with poorer oocyte quality, involving higher aneuploidy rates and decreased developmental competence. Prior to resumption of meiosis, fully developed mammalian oocytes become transcriptionally silent until the onset of zygotic genome activation. Therefore, meiotic progression and early embryogenesis are driven largely by translational utilization of previously synthesized mRNAs. We report that genome-wide translatome profiling reveals considerable numbers of transcripts that are differentially translated in oocytes obtained from aged compared to young females. Additionally, we show that a number of aberrantly translated mRNAs in oocytes from aged females are associated with cell cycle. Indeed, we demonstrate that four specific maternal age-related transcripts (Sgk1,Castor1,AireandEg5) with differential translation rates encode factors that are associated with the newly forming meiotic spindle. Moreover, we report substantial defects in chromosome alignment and cytokinesis in the oocytes of young females, in which candidate CASTOR1 and SGK1 protein levels or activity are experimentally altered. Our findings indicate that improper translation of specific proteins at the onset of meiosis contributes to increased chromosome segregation problems associated with female ageing. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2021 Electronic address https://onlinelibrary.wiley.com/doi/full/10.1111/acel.13231
Number of the records: 1