Number of the records: 1
Structural Variations Affecting Genes and Transposable Elements of Chromosome 3B in Wheats
- 1.
SYSNO ASEP 0532724 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Structural Variations Affecting Genes and Transposable Elements of Chromosome 3B in Wheats Author(s) De Oliveira, R. (FR)
Rimbert, H. (FR)
Balfourier, F. (FR)
Kitt, J. (FR)
Dynomant, E. (FR)
Vrána, Jan (UEB-Q) RID, ORCID
Doležel, Jaroslav (UEB-Q) RID, ORCID
Cattonaro, F. (IT)
Paux, E. (FR)
Choulet, F. (FR)Number of authors 10 Article number 891 Source Title Frontiers in genetics. - : Frontiers Media
Roč. 11, AUG 18 (2020)Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords bread wheat ; copy number variations ; genome evolution ; structural variations ; transposable elements ; Triticum Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000827 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000567928000001 EID SCOPUS 85090026615 DOI 10.3389/fgene.2020.00891 Annotation Structural variations (SVs) such as copy number and presence–absence variations are polymorphisms that are known to impact genome composition at the species level and are associated with phenotypic variations. In the absence of a reference genome sequence, their study has long been hampered in wheat. The recent production of new wheat genomic resources has led to a paradigm shift, making possible to investigate the extent of SVs among cultivated and wild accessions. We assessed SVs affecting genes and transposable elements (TEs) in a Triticeae diversity panel of 45 accessions from seven tetraploid and hexaploid species using high-coverage shotgun sequencing of sorted chromosome 3B DNA and dedicated bioinformatics approaches. We showed that 23% of the genes are variable within this panel, and we also identified 330 genes absent from the reference accession Chinese Spring. In addition, 60% of the TE-derived reference markers were absent in at least one accession, revealing a high level of intraspecific and interspecific variability affecting the TE space. Chromosome extremities are the regions where we observed most of the variability, confirming previous hypotheses made when comparing wheat with the other grasses. This study provides deeper insights into the genomic variability affecting the complex Triticeae genomes at the intraspecific and interspecific levels and suggests a phylogeny with independent hybridization events leading to different hexaploid species. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.3389/fgene.2020.00891
Number of the records: 1