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Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics
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SYSNO ASEP 0532623 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Photon-upconversion barcode for monitoring an enzymatic reaction with a fluorescence reporter in droplet microfluidics Author(s) Hlaváček, Antonín (UIACH-O) ORCID
Křivánková, Jana (UIACH-O) RID, ORCID
Pizúrová, Naděžda (UFM-A) RID, ORCID
Václavek, Tomáš (UIACH-O) ORCID, SAI
Foret, František (UIACH-O) RID, ORCIDNumber of authors 5 Source Title Analyst. - : Royal Society of Chemistry - ISSN 0003-2654
Roč. 145, č. 23 (2020), s. 7718-7723Number of pages 6 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords photon-upconversion ; droplet microfluidics ; fluorescence signal Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Subject RIV - cooperation Institute of Physics of Materials - Other Materials R&D Projects GJ18-03367Y GA ČR - Czech Science Foundation (CSF) 8F17003 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 ; UFM-A - RVO:68081723 UT WOS 000592315100026 EID SCOPUS 85096815551 DOI 10.1039/D0AN01667E Annotation We report luminescent photon-upconversion barcodes for indexing the chemical content of droplets. The barcode is compatible with the simultaneous detection of fluorescence. The encoding and decoding of the initial concentration of enzyme β-galactosidase and substrate 4-methylumbelliferyl β-D-galactopyranoside are described. The fluorescent product 4-methylumbelliferone is detected simultaneously with the barcode. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2021 Electronic address http://hdl.handle.net/11104/0311057
Number of the records: 1