Number of the records: 1
Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. Under arsenic stress
- 1.
SYSNO ASEP 0531600 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. Under arsenic stress Author(s) Pavlíková, D. (CZ)
Zemanová, Veronika (UEB-Q) ORCID, RID
Pavlík, Milan (UEB-Q) RID, ORCID
Dobrev, Petre (UEB-Q) RID, ORCID
Hnilička, F. (CZ)
Motyka, Václav (UEB-Q) RID, ORCIDNumber of authors 6 Article number e0233055 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 15, č. 5 (2020)Number of pages 22 s. Language eng - English Country US - United States Keywords FREE AMINO-ACIDS ; MAIZE BETA-GLUCOSIDASE ; TRANS-ZEATIN CONTENT Subject RIV DK - Soil Contamination ; De-contamination incl. Pesticides OECD category Biochemical research methods R&D Projects GA17-10591S GA ČR - Czech Science Foundation (CSF) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000537496000045 EID SCOPUS 85084785357 DOI 10.1371/journal.pone.0233055 Annotation Given the close relationship between cytokinins (CKs), photosynthesis and nitrogen metabolism, this study assessed the effect of arsenic (As) contamination on these metabolic components in the As-hyperaccumulators Pteris cretica L. var. Albo-lineata (Pc-A) and var. Parkerii (Pc-P) as well as the As-non-hyperaccumulator Pteris straminea Mett. ex Baker (Ps). The ferns were cultivated in a pot experiment for 23 weeks in soil spiked with As at the levels 20 and 100 mg·kg-1. For the purpose of this study, the CKs were placed into five functionally different groups according to their structure and physiological roles: bioactive forms (bCKs, CK free bases), inactive or weakly active forms (dCKs, CK N-glucosides), transport forms (tCKs, CK ribosides), storage forms (sCKs, O-glucosides), and primary products of CK biosynthesis (ppbCKs, CK nucleotides). An important finding was higher CKs total content, accumulation of sCKs and reduction of dCKs in As-hyperaccumulators in contrast to non-hyperaccumulator ferns. A significant depletion of C resources was confirmed in ferns, especially Ps, which was determined by measuring the photosynthetic rate and chlorophyll fluorescence. A fluorescence decrease signified a reduction in the C/N ratio, inducing an increase of bioactive CKs forms in Pc-P and Ps. The impact of As on N utilization was significant in As-hyperaccumulators. The glutamic acid/glutamine ratio, an indicator of primary N assimilation, diminished in all ferns with increased As level in the soil. In conclusion, the results indicate a large phenotypic diversity of Pteris species to As and suggest that the CKs composition and the glutamic acid/glutamine ratio can be used as a tool to diagnose As stress in plants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.1371/journal.pone.0233055
Number of the records: 1