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Electrographic seizures induced by activation of ETA and ETB receptors following intrahippocampal infusion of endothelin-1 in immature rats occur by different mechanisms
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SYSNO ASEP 0524259 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Electrographic seizures induced by activation of ETA and ETB receptors following intrahippocampal infusion of endothelin-1 in immature rats occur by different mechanisms Author(s) Vondráková, Kateřina (FGU-C) RID, ORCID, SAI
Novotný, P. (CZ)
Kubová, Hana (FGU-C) RID, ORCID
Pošusta, Antonín (FGU-C) RID
Boron, J. (CZ)
Fáberová, Veronika (FGU-C) ORCID, RID
Fabene, P. F. (IT)
Burchfiel, J. (US)
Tsenov, Grygoriy (FGU-C) RID, ORCIDArticle number 113255 Source Title Experimental Neurology. - : Elsevier - ISSN 0014-4886
Roč. 328, Jun (2020)Number of pages 9 s. Language eng - English Country US - United States Keywords ischemia ; hippocampus ; immature rat ; endothelin ; microdialysis, infmammatory ; mass spektrometry ; glutamate ; GABA ; leukotrienes ; receptors ; EEG ; seizures Subject RIV FH - Neurology OECD category Neurosciences (including psychophysiology R&D Projects GA18-09296S GA ČR - Czech Science Foundation (CSF) GA14-20613S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support FGU-C - RVO:67985823 UT WOS 000527888300009 EID SCOPUS 85080116749 DOI 10.1016/j.expneurol.2020.113255 Annotation Using microdialysis we to determine biomarkers associated with seizures in P12 rats following the intrahippocampal infusion of two different agents: (1) ET-1, which activates both the ETA and ETB receptors and causes focal ischemia and (2) Ala-ET-1, which selectively activates only the ETB receptor and does not cause ischemia.
Our results show that seizures associated with combined ETA and ETB receptor activation (and ischemia) have a different temporal distribution and microdialysis profile from seizures associated with ETB activation alone (and without ischemia). Seizures induced with ETA and ETB activation peak within the first hour after infusion and the microdialysis profile is characterized by a significant increase in the ratio of glutamic acid to GABA. By contrast,
seizures with activation of only the ETB receptor peak in the second hour after infusion and microdialysis shows a significant increase in the ratio of leukotriene B4 to prostaglandin E2.
These findings suggest that ischemia-related seizures in immature animals involve an imbalance of excitation and inhibition, while non-ischemia related seizures involve an inflammatory process resulting from an excess of leukotrienes.
Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2021 Electronic address https://doi.org/10.1016/j.expneurol.2020.113255
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