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MICRONUCLEI LEVELS IN NANOCOMPOSITES PRODUCTION WORKERS: INTERPRETATION OF RESULTS FROM TWO YEARS OF MONITORING
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SYSNO ASEP 0522981 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title MICRONUCLEI LEVELS IN NANOCOMPOSITES PRODUCTION WORKERS: INTERPRETATION OF RESULTS FROM TWO YEARS OF MONITORING Author(s) Rössnerová, Andrea (UEM-P) RID
Pelcová, D. (CZ)
Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
Rössner ml., Pavel (UEM-P) RID, ORCID
Elzeinova, Fatima (UEM-P)
Vrbová, Kristýna (UEM-P)
Topinka, Jan (UEM-P) RID, ORCID
Schwarz, Jaroslav (UCHP-M) RID, ORCID, SAI
Ondráček, Jakub (UCHP-M) RID, ORCID, SAI
Koštejn, Martin (UCHP-M) RID, SAI, ORCID
Komarc, M. (CZ)
Vlčková, Š. (CZ)
Fenclová, Z. (CZ)
Lischková, L. (CZ)
Dvořáčková, Š. (CZ)Source Title NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application. - Ostrava : Tanger Ltd., 2019 - ISBN 978-808729489-5 Pages s. 554-559 Number of pages 6 s. Publication form Print - P Action NANOCON 2018 -International Conference on Nanomaterials - Research and Application /10./ Event date 17.10.2018 - 19.10.2018 VEvent location Brno Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords genotoxicity ; human ; micronucleus assay Subject RIV DN - Health Impact of the Environment Quality OECD category Public and environmental health Subject RIV - cooperation Institute of Chemical Process Fundamentals - Health Impact of the Environment Quality R&D Projects LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA18-02079S GA ČR - Czech Science Foundation (CSF) Institutional support UEM-P - RVO:68378041 ; UCHP-M - RVO:67985858 UT WOS 000513131900096 EID SCOPUS 85062991930 Annotation Micronucleus assay, which has been used for almost sixty years, is one of the basic methodological approaches of genetic toxicology. This is also evident from more than 10,500 references found in PubMed database. These studies concern the evaluation of cytotoxicity and genotoxicity of numerous chemicals and in the last decade also nanomaterials (NM) and nanoparticles (NP). Surprisingly, no relevant human studies focused on MN formation following the exposure to NP have been reported.
In our study we analyzed 4x (in September 2016 and 2017, pre-shift and post-shift each year) samples in a group of workers, working for almost 18 years in nanocomposites research, and matched controls. Detail aerosol exposure monitoring of particulate matter (PM) including nano-sized fractions was completed during working shift in a sampling day. The micronucleus assay using Pan-Centromeric Chromosome Paint was applied to recognize, beside the frequency of total micronuclei (MN) in binucleated cells (BNC), also other types of chromosomal damage (losses and breaks), including the centromere positive (CEN+) and centromere negative (CEN-) micronuclei.
The monitoring data showed differences in the risk of exposure to NP related to individual working processes, as well as differences in chemical composition of nano-fraction. Cytogenetic results demonstrated consistently (both years): (i) possible adaptation to long-term exposure of NP (related to total frequency of MN), (ii) short-term (2.5 h) exposure could be a reason for the aberration increase, particularly a chromosomal losses (aneugenic effect).Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2020
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