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Toll-like receptor 2 expression on c-kit(+) cells tracks the emergence of embryonic definitive hematopoietic progenitors
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SYSNO ASEP 0520652 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Toll-like receptor 2 expression on c-kit(+) cells tracks the emergence of embryonic definitive hematopoietic progenitors Author(s) Balounová, Jana (UMG-J)
Šplíchalová, Iva (UMG-J)
Dobešová, Martina (UMG-J)
Kolář, Michal (UMG-J) RID, ORCID
Fišer, K. (CZ)
Procházka, Jan (UMG-J) ORCID
Sedláček, Radislav (UMG-J) RID
Jurisicova, A. (CA)
Sung, H.-k. (CA)
Kořínek, Vladimír (UMG-J) RID
Alberich-Jorda, Meritxell (UMG-J) RID
Godin, I. (FR)
Filipp, Dominik (UMG-J) RIDNumber of authors 13 Article number 5176 Source Title Nature Communications. - : Nature Publishing Group
Roč. 10, č. 1 (2019)Number of pages 14 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords tissue-resident macrophages ; stem-cells ; yolk-sac ; blood-cells ; in-vivo ; recognition ; circulation ; populations ; generation ; originate Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology R&D Projects LM2015040 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA19-23154S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0395 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UMG-J - RVO:68378050 UT WOS 000496713100007 DOI 10.1038/s41467-019-13150-0 Annotation Hematopoiesis in mammalian embryos proceeds through three successive waves of hematopoietic progenitors. Since their emergence spatially and temporally overlap and phenotypic markers are often shared, the specifics regarding their origin, development, lineage restriction and mutual relationships have not been fully determined. The identification of wave-specific markers would aid to resolve these uncertainties. Here, we show that toll-like receptors (TLRs) are expressed during early mouse embryogenesis. We provide phenotypic and functional evidence that the expression of TLR2 on E7.5 c-kit(+) cells marks the emergence of precursors of erythro-myeloid progenitors (EMPs) and provides resolution for separate tracking of EMPs from primitive progenitors. Using in vivo fate mapping, we show that at E8.5 the Tlr2 locus is already active in emerging EMPs and in progenitors of adult hematopoietic stem cells (HSC). Together, this data demonstrates that the activation of the Tlr2 locus tracks the earliest events in the process of EMP and HSC specification. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2020 Electronic address https://www.nature.com/articles/s41467-019-13150-0
Number of the records: 1