Crystal structures of Trypanosoma brucei hypoxanthine - guanine - xanthine phosphoribosyltransferase in complex with IMP, GMP and XMP

0519629 - BC 2020 RIV GB eng J - Journal Article
Terán, D. - Doleželová, Eva - Keough, D. T. - Hocková, Dana - Zíková, Alena - Guddat, L. W.
Crystal structures of Trypanosoma brucei hypoxanthine - guanine - xanthine phosphoribosyltransferase in complex with IMP, GMP and XMP.
FEBS Journal. Roč. 286, č. 23 (2019), s. 4721-4736. ISSN 1742-464X. E-ISSN 1742-4658
R&D Projects: GA ČR(CZ) GA19-07707S; GA MŠMT(CZ) EF16_019/0000759
Institutional support: RVO:60077344 ; RVO:61388963
Keywords : purine salvage * 6-oxopurine phosphoribosyltransferase * tritrichomonas-fetus * drug-resistance * pyrophosphate * enzyme * state * inhibitors * mutations * cloning * 6-oxopurine phosphoribosyltransferase * gmp * imp * purine salvage * Trypanosoma brucei * xmp
OECD category: Organic chemistry; Organic chemistry (UOCHB-X)
Impact factor: 4.392, year: 2019
Method of publishing: Limited access
https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/febs.14987

The 6-oxopurine phosphoribosyltransferases (PRTs) are drug targets for the treatment of parasitic diseases. This is due to the fact that parasites are auxotrophic for the 6-oxopurine bases relying on salvage enzymes for the synthesis of their 6-oxopurine nucleoside monophosphates. In Trypanosoma brucei, the parasite that is the aetiological agent for sleeping sickness, there are three 6-oxopurine PRT isoforms. Two are specific for hypoxanthine and guanine, whilst the third, characterized here, uses all three naturally occurring bases with similar efficiency. Here, we have determined crystal structures for TbrHGXPRT in complex with GMP, XMP and IMP to investigate the structural basis for substrate specificity. The results show that Y201 and E208, not commonly observed within the purine binding pocket of 6-oxopurine PRTs, contribute to the versatility of this enzyme. The structures further show that a nearby water can act as an adaptor to facilitate the binding of XMP and GMP. When GMP binds, a water can accept a proton from the 2-amino group but when XMP binds, the equivalent water can donate its proton to the 2-oxo group. However, when IMP is bound, no water molecule is observed at that location. Database Coordinates and structure factors were submitted to the Protein Data Bank and have accession codes of 6MXB, 6MXC, 6MXD and 6MXG for the TbrHGXPRT.XMP complex, TbrHGXPRT.GMP complex, TbrHGXPRT.IMP complex, and TbrHGPRT.XMP complex, respectively.
Permanent Link: http://hdl.handle.net/11104/0304637