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Detection of Single Nucleotide Polymorphisms Using Selective Incorporation of Biotin in DNA Strand and Subsequent Enzymatic Detection at Pencil Electrode
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SYSNO ASEP 0506761 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Detection of Single Nucleotide Polymorphisms Using Selective Incorporation of Biotin in DNA Strand and Subsequent Enzymatic Detection at Pencil Electrode Author(s) Plucnara, Medard (BFU-R) ORCID
Ecsin, E. (TR)
Erdem, A. (TR)
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 4 Source Title XXXIV. Moderní elektrochemické metody sborník přednášek. - Ústí nad Labem : Best servis, 2014 / Navrátil T. ; Fojta M. ; Pecková K. - ISBN 978-80-905221-2-1 Pages č. 2014 (2014), s. 131-134 Number of pages 4 s. Publication form Print - P Action Moderní Elektrochemické Metody /34./ Event date 19.05.2014-23.05.2014 VEvent location Jetřichovice Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords Single nucleotide polymorphism ; Enzymatic labeling ; Pencil graphite electrode Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GBP206/12/G151 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000353446900028 Annotation Enzyme-linked electrochemical assay using DNA labeling by biotin followed by binding of enzyme-streptavidin conjugates has been found to be a potent tool for DNA diagnostic. This approach brings a special advantage of signal amplification due to the fact, that only very small number of enzymatic labels can produce a number of molecules of an electrochemically detectable product from an inactive substrate to obtain sufficiently strong signal. A new way of using of this technique combined with selective primer extension reaction designed for the detection of single nucleotide polymorphism is presented here. The assay was combined with measurements at a pencil graphite electrode, which is a very practical tool for potential clinical applications due to its cheapness and disposability. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020
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